4-Fluoro-7-nitrobenzofurazan

Modify Date: 2024-01-11 16:24:04

4-Fluoro-7-nitrobenzofurazan Structure
4-Fluoro-7-nitrobenzofurazan structure
Common Name 4-Fluoro-7-nitrobenzofurazan
CAS Number 29270-56-2 Molecular Weight 183.097
Density 1.7±0.1 g/cm3 Boiling Point 308.6±45.0 °C at 760 mmHg
Molecular Formula C6H2FN3O3 Melting Point 52-54 °C(lit.)
MSDS Chinese USA Flash Point 140.4±28.7 °C
Symbol GHS07
GHS07
Signal Word Warning

 Use of 4-Fluoro-7-nitrobenzofurazan


NBD-F is a fluorescent derivatization reagent which is originally developed for amino acid analysis.

 Names

Name 4-fluoro-7-nitro-2,1,3-benzoxadiazole
Synonym More Synonyms

 4-Fluoro-7-nitrobenzofurazan Biological Activity

Description NBD-F is a fluorescent derivatization reagent which is originally developed for amino acid analysis.
Related Catalog
In Vitro As the percentage of the organic phase changes, the retention time of NBD-F remains relatively stable, while the retention times of the derivatization products changes. The pH of the mobile phase affects the separation of the NBD-F and the derivatization products[1]. NBD-F is a fluorescent derivatization reagent that is originally developed for amino acid analysis, and recently applied to the analysis of other amino acid derivatives such as N-methyl-D-aspartic acid and glutathione. The use of NBD-F appears to have several advantages in that the derivatization procedure is simple and its derivatives are highly stable[2].
Kinase Assay An accurately weighed quantity (0.0183 g) of NBD-F is transferred into a 1 mL centrifuge tube, dissolved in acetonitrile and made up to volume with the same solvent to produce stock solutions of 0.1 M. The solution is protected from light and stored at -20°C until analyzed. A 100 μL aliquot of mixed amino acids solution or sample supernatant, 175 μL of borate buffer solution, 200 μL of acetonitrile and 25 μL of NBD-F working solution are mixed in a 1.5 mL centrifuge tube. The well-mixed solution is allowed to react at 60°C in the water bath for 7 min, excluding light. NBD-F reacts with amino group and enables amino acids to be detected with UV detection. After cooling to room temperature, 10 μL of the solution is injected into the equilibrated HPLC system[1].
References

[1]. Wu X, et al. Determination of amino acid neurotransmitters in rat hippocampi by HPLC-UV using NBD-F as a derivative. Biomed Chromatogr. 2014 Apr;28(4):459-62.

[2]. Ishikawa T, et al. Development of an LC-MS/MS method for the analysis of free sphingoid bases using 4-fluoro-7-nitrobenzofurazan (NBD-F). Lipids. 2014 Mar;49(3):295-304.

 Chemical & Physical Properties

Density 1.7±0.1 g/cm3
Boiling Point 308.6±45.0 °C at 760 mmHg
Melting Point 52-54 °C(lit.)
Molecular Formula C6H2FN3O3
Molecular Weight 183.097
Flash Point 140.4±28.7 °C
Exact Mass 183.008026
PSA 84.74000
LogP 1.27
Vapour Pressure 0.0±0.6 mmHg at 25°C
Index of Refraction 1.638
Storage condition 2-8°C

 Safety Information

Symbol GHS07
GHS07
Signal Word Warning
Hazard Statements H315-H319
Precautionary Statements P305 + P351 + P338
Personal Protective Equipment dust mask type N95 (US);Eyeshields;Gloves
Hazard Codes Xi: Irritant;
Risk Phrases R36/38
Safety Phrases S26-S36-S39
RIDADR NONH for all modes of transport
WGK Germany 3
HS Code 2934999090

 Synthetic Route

~%

4-Fluoro-7-nitrobenzofurazan Structure

4-Fluoro-7-nitr...

CAS#:29270-56-2

Literature: Jung, Michael E.; Dong, Timothy A.; Cai, Xiaolu Tetrahedron Letters, 2011 , vol. 52, # 20 p. 2533 - 2535

~%

4-Fluoro-7-nitrobenzofurazan Structure

4-Fluoro-7-nitr...

CAS#:29270-56-2

Literature: Jung, Michael E.; Dong, Timothy A.; Cai, Xiaolu Tetrahedron Letters, 2011 , vol. 52, # 20 p. 2533 - 2535

~%

4-Fluoro-7-nitrobenzofurazan Structure

4-Fluoro-7-nitr...

CAS#:29270-56-2

Literature: Jung, Michael E.; Dong, Timothy A.; Cai, Xiaolu Tetrahedron Letters, 2011 , vol. 52, # 20 p. 2533 - 2535

 Customs

HS Code 2934999090
Summary 2934999090. other heterocyclic compounds. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0%

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 Synonyms

7-fluoro-4-nitrobenzofurazan
2,1,3-Benzoxadiazole, 4-fluoro-7-nitro-
4-fluoro-7-nitrobenz-2-oxa-1,3-diazole
NBD-F
BENZOFURAZAN, 4-FLUORO-7-NITRO-
7-Fluoro-4-nitrobenzo-2-oxa-1,3-diazole
4-fluoro-7-nitro-2,1,3-benzooxadiazole
4-Fluoro-7-nitro-2,1,3-benzoxadiazole
MFCD00010196
4-Fluoro-7-nitrobenzofurazan
(4-Fluoro-7-nitro-2,1,3-benzoxadiazole)
NBD fluoride