KN-93 hydrochloride
Modify Date: 2024-01-10 19:36:21
KN-93 hydrochloride structure
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Common Name | KN-93 hydrochloride | ||
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| CAS Number | 1956426-56-4 | Molecular Weight | 537.498 | |
| Density | N/A | Boiling Point | N/A | |
| Molecular Formula | C26H30Cl2N2O4S | Melting Point | N/A | |
| MSDS | N/A | Flash Point | N/A | |
Use of KN-93 hydrochlorideKN-93 hydrochloride is a cell-permeable, reversible and competitive inhibitor calmodulin-dependent kinase type II (CaMKII) with a Ki of 370 nM. |
| Name | KN-93 hydrochloride |
|---|---|
| Synonym | More Synonyms |
| Description | KN-93 hydrochloride is a cell-permeable, reversible and competitive inhibitor calmodulin-dependent kinase type II (CaMKII) with a Ki of 370 nM. |
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| Related Catalog | |
| Target |
Ki: 370 nM (CaMK-II) |
| In Vitro | After 2 days of KN-93 treatment, 95% of cells are arrested in G1. G1 arrest is reversible; 1 day after KN-93 release, a peak of cells had progressed into S and G2-M. KN-93 also blocks cell growth stimulated by basic fibroblast growth factor, platelet-derived growth factor-BB, epidermal growth factor, and insulin-like growth factor-1 in NIH 3T3 fibroblasts[1]. KN-93 inhibits the H+, K+-ATPase activity but strongly dissipates the proton gradient formed in the gastric membrane vesicles and reduces the volume of luminal space[2]. KN-93 (0.5 μM) prevents increased LV developed pressure during action potential prolongation and early afterdepolarizations. Ca2+-independent CaM kinase activity is increased during early afterdepolarizations and this increase is prevented by KN-93[3]. |
| Kinase Assay | Cells are grown on 12-mm diameter glass coverslips in DMEM 100% serum and various concentrations of KN-93 or KN-92. After 0, 1, 2, and 3 days of culture in the presence of drug, coverslips are removed from culture, rinsed once in PBS, and then submerged in 100% methanol at -20°C for 3 min. Fixed cells are stored in PBS until staining using the TUNEL assay. Cells are overlaid on 20 μL PBS/1 mg/mL BSA for 30 min, rinsed in PBS, and then overlaid on 20 μL containing 100 mM sodium cacodylate (pH 6.8), 1 mM CoCl2, 0.1 mM DTT, 0.1 mg/mL BSA, 20 μM fluorescein-12-dUTP, and 0.1 unit/μL terminal transferase at 37°C for 60 min. Coverslips are rinsed in PBS twice, mounted on slides, and photographed using an OLYMPUS BX5O epifluorescent microscope using a UPLAN APO 40X oil immersion objective. |
| References |
| Molecular Formula | C26H30Cl2N2O4S |
|---|---|
| Molecular Weight | 537.498 |
| Exact Mass | 536.130310 |
| Storage condition | 2-8℃ |
| MFCD27992054 |
| Benzenesulfonamide, N-[2-[[[(2E)-3-(4-chlorophenyl)-2-propen-1-yl]methylamino]methyl]phenyl]-N-(2-hydroxyethyl)-4-methoxy-, hydrochloride (1:1) |
| N-[2-({[(2E)-3-(4-Chlorophenyl)-2-propen-1-yl](methyl)amino}methyl)phenyl]-N-(2-hydroxyethyl)-4-methoxybenzenesulfonamide hydrochloride (1:1) |
| KN-93 (hydrochloride) |