WST-1

Modify Date: 2025-09-07 17:19:20

WST-1 Structure
WST-1 structure
Common Name WST-1
CAS Number 150849-52-8 Molecular Weight 651.34400
Density N/A Boiling Point N/A
Molecular Formula C19H11IN5NaO8S2 Melting Point > 300°C (dec.)
MSDS N/A Flash Point N/A

 Use of WST-1


WST-1 is a Tetrazolium dye, widely used in the cell viability assays. WST-1 produces a water-soluble formazan product (WST-1 formazan) that occurs at the cell surface in NADPH dependent manner in viable cells[1][2].

 Names

Name sodium,4-[2-(4-iodophenyl)-3-(4-nitrophenyl)tetrazol-2-ium-5-yl]benzene-1,3-disulfonate
Synonym More Synonyms

 WST-1 Biological Activity

Description WST-1 is a Tetrazolium dye, widely used in the cell viability assays. WST-1 produces a water-soluble formazan product (WST-1 formazan) that occurs at the cell surface in NADPH dependent manner in viable cells[1][2].
Related Catalog
In Vitro WST-1 is used for a wide range of assays that include cell viability, proliferation, plasma membrane electron transport system, superoxide generation, superoxide dismutase, and xanthine oxidase assay[1]. A water-soluble formazan dye is produced from the reduction of WST-1 tetrazolium, of which optical density at 450 nm is measured to evaluate cell viability[1].
References

[1]. Kyung-Mi Joo, et al. Development and validation of UPLC method for WST-1 cell viability assay and its application to MCTT HCE™ eye irritation test for colorful substances. Toxicol In Vitro. 2019 Oct;60:412-419.

[2]. Munetaka ISHIYAMA, et al. A new sulfonated tetrazolium salt that produces a highly water-soluble formazan dye. Chem. Pharm. Bull. 1993, 41(6): 1118-1122.

 Chemical & Physical Properties

Melting Point > 300°C (dec.)
Molecular Formula C19H11IN5NaO8S2
Molecular Weight 651.34400
Exact Mass 650.89900
PSA 211.57000
LogP 4.21680
InChIKey JUJBNYBVVQSIOU-UHFFFAOYSA-M
SMILES O=[N+]([O-])c1ccc(-[n+]2nc(-c3ccc(S(=O)(=O)[O-])cc3S(=O)(=O)[O-])nn2-c2ccc(I)cc2)cc1.[Na+]
Storage condition -20°C

 Safety Information

Hazard Codes C

 Synonyms

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wst-1
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Price: ¥550/10 mM * 1 mL in DMSO

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