WST-8

Modify Date: 2025-08-25 16:42:23

Use of WST-8

WST-8 is a water-soluble tetrazolium dye, WST-8 enhances sensitivity of the WST-8-based assay over the conventional MTS-based assay.

Name	sodium,4-[2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)tetrazol-2-ium-5-yl]benzene-1,3-disulfonate
Synonym	More Synonyms

Description	WST-8 is a water-soluble tetrazolium dye, WST-8 enhances sensitivity of the WST-8-based assay over the conventional MTS-based assay.
Related Catalog	Signaling Pathways >> Others >> Others Biochemical Assay Reagents Research Areas >> Others
In Vitro	The generally used MTS-based assay is compared with a bioassay employing a water-soluble tetrazolium dye, WST-8, using NFS-60 cells at a concentration of 7×105 cells/mL against 800 IU/mL of PEGylated G-CSF at 24, 48, 72, and 72 h time points to determine the efficacy of PEGylated G-CSF. Further, the optimized WST-8 dye-based assay is used to test the potency of various commercially available PEGylated G-CSF preparations. The results demonstrate enhanced sensitivity of the WST-8-based assay over the conventional MTS-based assay for determining the potency of PEGylated G-CSF using the NFS-60 cell line[1].
References	[1]. Tiwari K, et al. A sensitive WST-8-based bioassay for PEGylated granulocyte colony stimulating factor using the NFS-60 cell line. Pharm Biol. 2015 Jun;53(6):849-54.

Molecular Formula	C₂₀H₁₄N₆NaO₁₁S₂
Molecular Weight	601.48
PSA	266.62000
LogP	4.05220
InChIKey	VSIVTUIKYVGDCX-UHFFFAOYSA-M
SMILES	COc1cc([N+](=O)[O-])ccc1-[n+]1nc(-c2ccc(S(=O)(=O)[O-])cc2S(=O)(=O)[O-])nn1-c1ccc([N+](=O)[O-])cc1.[Na+]
Storage condition	2~8°C

Hazard Codes	C

Sodium 4-[3-(2-methoxy-4-nitrophenyl)-2-(4-nitrophenyl)-2H-tetrazol-3-ium-5-yl]-1,3-benzenedisulfonate

wst-8

2H-Tetrazolium, 5-(2,4-disulfophenyl)-3-(2-methoxy-4-nitrophenyl)-2-(4-nitrophenyl)-, inner salt, sodium salt (1:1)

Dayang Chem (Hangzhou) Co., Ltd.
China
Product Name: 2-(2-Methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium sodium salt
Price: $Inquiry/100g $Inquiry/1kg $Inquiry/100kg $Inquiry/1000kg
Purity: 98.0%
Delivery: Inquiry
Contact: Ms Wang
Email: enquiry@dycnchem.com

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Price: $66/10mM*1mLinWater

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