6-Chloro-1H-indol-3-yl β-D-galactopyranoside
Modify Date: 2024-02-01 11:35:12
6-Chloro-1H-indol-3-yl β-D-galactopyranoside structure
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Common Name | 6-Chloro-1H-indol-3-yl β-D-galactopyranoside | ||
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| CAS Number | 138182-21-5 | Molecular Weight | 329.733 | |
| Density | 1.6±0.1 g/cm3 | Boiling Point | 630.2±55.0 °C at 760 mmHg | |
| Molecular Formula | C14H16ClNO6 | Melting Point | N/A | |
| MSDS | N/A | Flash Point | 335.0±31.5 °C | |
Use of 6-Chloro-1H-indol-3-yl β-D-galactopyranosideRose-β-D-Gal is a flurescent dye, is also a β-galactosidase substrate. Rose-β-D-Gal creates a pink/magenta color after the reaction and has been used for detection of β-gal activity[1][2]. |
| Name | 6-Chloro-1H-indol-3-yl-β-D-galactopyranoside |
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| Synonym | More Synonyms |
| Description | Rose-β-D-Gal is a flurescent dye, is also a β-galactosidase substrate. Rose-β-D-Gal creates a pink/magenta color after the reaction and has been used for detection of β-gal activity[1][2]. |
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| Related Catalog | |
| In Vitro | Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).Immunostaining of Tissues[1][2]: 1.Prepare 25 mg/mL Rose-β-D-Gal stock solution in Dimethyl Sulfoxide (DMSO) and store at -20 °C. It is vital to protect the stock solution from the light. 2.Prepare rinse buffer for Rose-β-D-Gal staining: 0.1 % Sodium Deoxycholate, 0.2 % IGEPAL CA-630, 2 mM MgCl2 in 0.1 M Na Phosphate buffer (pH 7.3). Prepare 1,000 mL of 0.5 M Na Phosphate buffer (pH 7.3) by mixing with 158 mL of 1 M NaH2PO4, 342 mL of 1 M Na 2HPO4, and 500 mL water. 3.Prepare substrate solution for Rose-β-D-Gal fresh, containing 1 mg/mL Rose-β-D-Gal, 5 mM Potassium Ferricyanide, and 5 mM Potassium Ferrocyanide in the rinse buffer. 4.Rehydrate frozen tissue sections in PBS: quickly wash with PBS twice, wash with rinse buffer for 10 min 3 time. 5.Expose to β-galactosidase substrate Rose-β-D-Gal: stain at 37 ℃ for as long as needed to see stain, up to an overnight time period, keep covered and in the dark during color development. Wash with PBS for 5 min twice. 6.Fix tissue in 4% PFA, unless on intend to continue with in situ hybridizations, and continue to step 4. Note: Rose-β-D-Gal is not very stable in alcohols or organic solvents, so must use water-based counterstains such as Gill’s hematoxylin and cover slipping with aqueous mountant. |
| References |
[1]. Ismail J A , et al. Immunohistologic labeling of murine endothelium[J]. 2003, 12(2):0-90. |
| Density | 1.6±0.1 g/cm3 |
|---|---|
| Boiling Point | 630.2±55.0 °C at 760 mmHg |
| Molecular Formula | C14H16ClNO6 |
| Molecular Weight | 329.733 |
| Flash Point | 335.0±31.5 °C |
| Exact Mass | 329.066620 |
| PSA | 115.17000 |
| LogP | -0.15 |
| Vapour Pressure | 0.0±1.9 mmHg at 25°C |
| Index of Refraction | 1.717 |
| Storage condition | 2-8°C |
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6-Chloro-3-indolyl β-D-Galactopyranoside [for
Revision number: 5
Biochemical Research] SAFETY DATA SHEET Section1. IDENTIFICATION 6-Chloro-3-indolyl β-D-Galactopyranoside [for Biochemical Research] Product name: Revision number:5 Section2. HAZARDS IDENTIFICATION GHS classification Not classified PHYSICAL HAZARDS HEALTH HAZARDSNot classified Not classified ENVIRONMENTAL HAZARDS GHS label elements, including precautionary statements None Pictograms or hazard symbols Signal wordNo signal word None Hazard statements Precautionary statements:None Section3. COMPOSITION/INFORMATION ON INGREDIENTS Substance/mixture:Substance Components:6-Chloro-3-indolyl β-D-Galactopyranoside [for Biochemical Research] Percent:>98.0%(LC) CAS Number:138182-21-5 Synonyms:Salmon-Gal , Rose-Gal C14H16ClNO6 Chemical Formula: Section4. FIRST AID MEASURES Inhalation:Remove victim to fresh air and keep at rest in a position comfortable for breathing. Get medical advice/attention if you feel unwell. Skin contact:Remove/Take off immediately all contaminated clothing. Rinse skin with water/shower. If skin irritation or rash occurs: Get medical advice/attention. Eye contact:Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. If eye irritation persists: Get medical advice/attention. Ingestion:Get medical advice/attention if you feel unwell. Rinse mouth. A rescuer should wear personal protective equipment, such as rubber gloves and air- Protection of first-aiders: tight goggles. Section5. FIRE-FIGHTING MEASURES Suitable extinguishingDry chemical, foam, water spray, carbon dioxide. media: Specific hazards arising Take care as it may decompose upon combustion or in high temperatures to from the chemical:generate poisonous fume. Biochemical Research] Section5. FIRE-FIGHTING MEASURES Precautions for firefighters: Fire-extinguishing work is done from the windward and the suitable fire-extinguishing method according to the surrounding situation is used. Uninvolved persons should evacuate to a safe place. In case of fire in the surroundings: Remove movable containers if safe to do so. When extinguishing fire, be sure to wear personal protective equipment. Special protective equipment for firefighters: Section6. ACCIDENTAL RELEASE MEASURES Personal precautions,Use personal protective equipment. Keep people away from and upwind of spill/leak. protective equipment and Entry to non-involved personnel should be controlled around the leakage area by emergency procedures: roping off, etc. Environmental precautions: Prevent product from entering drains. Methods and materials for Sweep dust to collect it into an airtight container, taking care not to disperse it. containment and cleaning Adhered or collected material should be promptly disposed of, in accordance with up: appropriate laws and regulations. Section7. HANDLING AND STORAGE Precautions for safe handling Technical measures:Handling is performed in a well ventilated place. Wear suitable protective equipment. Prevent dispersion of dust. Wash hands and face thoroughly after handling. Use a local exhaust if dust or aerosol will be generated. Advice on safe handling: Avoid contact with skin, eyes and clothing. Conditions for safe storage, including any incompatibilities Storage conditions:Keep container tightly closed. Store in a freezer. Store under inert gas. Store away from incompatible materials such as oxidizing agents. Heat-sensitive, Light-sensitive, Air-sensitive Packaging material:Comply with laws. Section8. EXPOSURE CONTROLS / PERSONAL PROTECTION Engineering controls:Install a closed system or local exhaust as possible so that workers should not be exposed directly. Also install safety shower and eye bath. Personal protective equipment Respiratory protection: Dust respirator. Follow local and national regulations. Hand protection:Protective gloves. Eye protection:Safety glasses. A face-shield, if the situation requires. Skin and body protection: Protective clothing. Protective boots, if the situation requires. Section9. PHYSICAL AND CHEMICAL PROPERTIES Solid Physical state (20°C): Form:Crystal- Powder Colour:White - Slightly pale yellow Odour:No data available pH: No data available Melting point/freezing point:No data available Boiling point/range:No data available No data available Flash point: Flammability or explosive limits: Lower:No data available No data available Upper: Relative density:No data available Solubility(ies): [Water]No data available [Other solvents]No data available Biochemical Research] Section10. STABILITY AND REACTIVITY Chemical stability:Stable under proper conditions. Possibility of hazardous No special reactivity has been reported. reactions: Incompatible materials: Oxidizing agents Hazardous decomposition Carbon monoxide, Carbon dioxide, Nitrogen oxides (NOx), Hydrogen chloride products: Section11. TOXICOLOGICAL INFORMATION Acute Toxicity:No data available Skin corrosion/irritation: No data available Serious eyeNo data available damage/irritation: Germ cell mutagenicity: No data available Carcinogenicity: IARC =No data available NTP =No data available Reproductive toxicity:No data available Section12. ECOLOGICAL INFORMATION Ecotoxicity: No data available Fish: Crustacea:No data available No data available Algae: Persistence / degradability: No data available No data available Bioaccumulative potential(BCF): Mobility in soil Log Pow:No data available No data available Soil adsorption (Koc): Henry's LawNo data available constant(PaM3/mol): Section13. DISPOSAL CONSIDERATIONS Recycle to process, if possible. Consult your local regional authorities. You may be able to dissolve or mix material with a combustible solvent and burn in a chemical incinerator equipped with an afterburner and scrubber system. Observe all federal, state and local regulations when disposing of the substance. Section14. TRANSPORT INFORMATION Hazards Class:Does not correspond to the classification standard of the United Nations Not listed UN-No: Section15. REGULATORY INFORMATION Safe management ordinance of dangerous chemical product (State Council announces on January 26, 2002 and revised on February 16,2011): Safe use and production, the storage of a dangerous chemical, transport, loading and unloading were prescribed. Biochemical Research] SECTION 16 - ADDITIONAL INFORMATION N/A |
| Hazard Codes | Xi |
|---|---|
| Risk Phrases | 36/37/38 |
| Safety Phrases | S24/25-S36-S26 |
| WGK Germany | 3 |
| Salmon-GalRose-Gal |
| SALMON GALACTOSIDE |
| ROSE-GAL |
| β-D-Galactopyranoside, 6-chloro-1H-indol-3-yl |
| 6-chloro-1H-indol-3-yl-b-D-galactopyranoside |
| 6-Chloro-3-indolyl β-D-Galactopyranoside |
| SALMON-GAL |
| SALMON GLUCOSIDE |
| SALMON GLU |
| ROSE(TM)-GAL |
| ROSE(TM)-GLUCOSIDE |
| MFCD00467206 |
| RED-GAL(R) |
| 6-Chloro-3-indolyl-beta-D-galactopyranoside |
| 6-Chloro-1H-indol-3-yl β-D-galactopyranoside |