6-Chloro-1H-indol-3-yl β-D-galactopyranoside

Names

[ Name ]:
6-Chloro-1H-indol-3-yl β-D-galactopyranoside

[Synonym ]:
Salmon-GalRose-Gal
SALMON GALACTOSIDE
ROSE-GAL
β-D-Galactopyranoside, 6-chloro-1H-indol-3-yl
6-chloro-1H-indol-3-yl-b-D-galactopyranoside
6-Chloro-3-indolyl β-D-Galactopyranoside
SALMON-GAL
SALMON GLUCOSIDE
SALMON GLU
ROSE(TM)-GAL
ROSE(TM)-GLUCOSIDE
MFCD00467206
RED-GAL(R)
6-Chloro-3-indolyl-beta-D-galactopyranoside
6-Chloro-1H-indol-3-yl β-D-galactopyranoside

Biological Activity

[Description]:

Rose-β-D-Gal is a flurescent dye, is also a β-galactosidase substrate. Rose-β-D-Gal creates a pink/magenta color after the reaction and has been used for detection of β-gal activity[1][2].

[Related Catalog]:

Research Areas >> Others

Signaling Pathways >> Others >> Others

[In Vitro]

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).Immunostaining of Tissues[1][2]: 1.Prepare 25 mg/mL Rose-β-D-Gal stock solution in Dimethyl Sulfoxide (DMSO) and store at -20 °C. It is vital to protect the stock solution from the light. 2.Prepare rinse buffer for Rose-β-D-Gal staining: 0.1 % Sodium Deoxycholate, 0.2 % IGEPAL CA-630, 2 mM MgCl2 in 0.1 M Na Phosphate buffer (pH 7.3). Prepare 1,000 mL of 0.5 M Na Phosphate buffer (pH 7.3) by mixing with 158 mL of 1 M NaH2PO4, 342 mL of 1 M Na 2HPO4, and 500 mL water. 3.Prepare substrate solution for Rose-β-D-Gal fresh, containing 1 mg/mL Rose-β-D-Gal, 5 mM Potassium Ferricyanide, and 5 mM Potassium Ferrocyanide in the rinse buffer. 4.Rehydrate frozen tissue sections in PBS: quickly wash with PBS twice, wash with rinse buffer for 10 min 3 time. 5.Expose to β-galactosidase substrate Rose-β-D-Gal: stain at 37 ℃ for as long as needed to see stain, up to an overnight time period, keep covered and in the dark during color development. Wash with PBS for 5 min twice. 6.Fix tissue in 4% PFA, unless on intend to continue with in situ hybridizations, and continue to step 4. Note: Rose-β-D-Gal is not very stable in alcohols or organic solvents, so must use water-based counterstains such as Gill’s hematoxylin and cover slipping with aqueous mountant.

[References]

[1]. Ismail J A , et al. Immunohistologic labeling of murine endothelium[J]. 2003, 12(2):0-90.

[2]. Komatsu Y, et al. In situ hybridization methods for mouse whole mounts and tissue sections with and without additional β-galactosidase staining. Methods Mol Biol. 2014;1092:1-15.

Chemical & Physical Properties

[ Density]:
1.6±0.1 g/cm3

[ Boiling Point ]:
630.2±55.0 °C at 760 mmHg

[ Molecular Formula ]:
C₁₄H₁₆ClNO₆

[ Molecular Weight ]:
329.733

[ Flash Point ]:
335.0±31.5 °C

[ Exact Mass ]:
329.066620

[ PSA ]:
115.17000

[ LogP ]:
-0.15

[ Vapour Pressure ]:
0.0±1.9 mmHg at 25°C

[ Index of Refraction ]:
1.717

[ Storage condition ]:
2-8°C

MSDS

Click to get detail MSDS

Safety Information

[ Hazard Codes ]:
Xi

[ Risk Phrases ]:
36/37/38

[ Safety Phrases ]:
S24/25-S36-S26

[ WGK Germany ]:
3