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20325-40-0

20325-40-0 structure
20325-40-0 structure
  • Name: 3,3'-Dimethoxybenzidine dihydrochloride
  • Chemical Name: 3,3'-Dimethoxybenzidine dihydrochloride
  • CAS Number: 20325-40-0
  • Molecular Formula: C14H18Cl2N2O2
  • Molecular Weight: 317.211
  • Catalog: analytical chemistry Analytical reagent Common analytical reagents
  • Create Date: 2018-07-19 11:49:46
  • Modify Date: 2025-08-21 16:39:32
  • o-Dianisidine dihydrochloride is a colorimetric peroxidase substrate suitable for use in ELISA procedures. The compound has been used as a substrate to measure the oxidase activity of copper. o-Dianisidine dihydrochloride has also been used for a paper based-microfluidic device to semi-quantitatively measure lactate, uric acid, and glucose.
Name 3,3'-Dimethoxybenzidine dihydrochloride
Synonyms [1,1'-Biphenyl]-4,4'-diamine, 3,3'-dimethoxy-, hydrochloride (1:2)
[1,1'-biphenyl]-4,4'-diamine, 3,3'-dimethoxy-, dihydrochloride
3,3'-Dimethoxybenzidine dihydrochloride
3,3'-Dimethoxy-4,4'-diaminobiphenyl dihydrochloride
3,3'-Dimethoxy-[1,1'-biphenyl]-4,4'-diamine dihydrochloride
MFCD00012488
3,3′-Dimethoxybenzidine dihydrochloride
3,3'-Dimethoxy-4,4'-biphenyldiamine dihydrochloride
DMB DIHYDROCHLORIDE
o-Dianisidinehydrochloride
O-DIANISIDINE REAGENT
BLUE BN BASE DIHYDROCHLORIDE
2-Dianisidine dihydrochloride
EINECS 243-737-5
3,3‘-Dimethoxybenzidine dihydrochloride
2-Dianisidinedihydrochloride
o-Dianisidine Dihydrochloride
3,3'-Dimethoxy-benzidin,Dihydrochlorid
2-Dianisidinedi2HCl
Fast Blue B
3,3'-dimethoxybiphenyl-4,4'-ylenediammonium dichloride
33DIMETHOXYBENZIDINE2HCL
3,3'-dimethoxy-benzidine,dihydrochloride
3,3'-dimethoxybiphenyl-4,4'-diamine dihydrochloride
o-Dianisidine dihydr
o-Dianisidine (dihydrochloride)
Description o-Dianisidine dihydrochloride is a colorimetric peroxidase substrate suitable for use in ELISA procedures. The compound has been used as a substrate to measure the oxidase activity of copper. o-Dianisidine dihydrochloride has also been used for a paper based-microfluidic device to semi-quantitatively measure lactate, uric acid, and glucose.
Related Catalog
References

[1]. Iskra M et al. Copper and zinc concentrations and the activities of ceruloplasmin and superoxide dismutase inatherosclerosis obliterans. Biol Trace Elem Res. 2000 Jan;73(1):55-65.

[2]. Dungchai W et al. Use of multiple colorimetric indicators for paper-based microfluidic devices. Anal Chim Acta. 2010 Aug 3;674(2):227-33.
Boiling Point 391.3ºC at 760 mmHg
Melting Point 268 °C
Molecular Formula C14H18Cl2N2O2
Molecular Weight 317.211
Flash Point 206℃
Exact Mass 316.074524
PSA 70.50000
LogP 5.30160
Water Solubility 1-5 g/100 mL at 20 ºC

CHEMICAL IDENTIFICATION

RTECS NUMBER :
DD1050000
CHEMICAL NAME :
Benzidine, 3,3'-dimethoxy-, dihydrochloride
CAS REGISTRY NUMBER :
20325-40-0
LAST UPDATED :
199701
DATA ITEMS CITED :
19
MOLECULAR FORMULA :
C14-H16-N2-O2.2Cl-H
MOLECULAR WEIGHT :
317.24
WISWESSER LINE NOTATION :
1OR BZ ER DZ CO1 &GH 2

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

TYPE OF TEST :
TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE :
Oral
SPECIES OBSERVED :
Rodent - rat
DOSE/DURATION :
9100 mg/kg/13W-C
TOXIC EFFECTS :
Liver - changes in liver weight Kidney, Ureter, Bladder - changes in bladder weight Blood - changes in serum composition (e.g. TP, bilirubin, cholesterol)
TYPE OF TEST :
TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE :
Oral
SPECIES OBSERVED :
Rodent - rat
DOSE/DURATION :
8271 mg/kg/13W-C
TOXIC EFFECTS :
Liver - changes in liver weight Kidney, Ureter, Bladder - changes in bladder weight Nutritional and Gross Metabolic - weight loss or decreased weight gain
TYPE OF TEST :
TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE :
Oral
SPECIES OBSERVED :
Rodent - rat
DOSE/DURATION :
9009 mg/kg/39W-C
TOXIC EFFECTS :
Blood - other changes Liver - changes in liver weight Kidney, Ureter, Bladder - changes in bladder weight
TYPE OF TEST :
TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE :
Oral
SPECIES OBSERVED :
Rodent - rat
DOSE/DURATION :
1040 mg/kg/1Y-I
TOXIC EFFECTS :
Tumorigenic - Carcinogenic by RTECS criteria Sense Organs and Special Senses (Ear) - effect, not otherwise specified Reproductive - Tumorigenic effects - testicular tumors
TYPE OF TEST :
TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE :
Oral
SPECIES OBSERVED :
Rodent - mouse
DOSE/DURATION :
5760 mg/kg/2Y-I
TOXIC EFFECTS :
Tumorigenic - equivocal tumorigenic agent by RTECS criteria Liver - tumors Skin and Appendages - tumors
TYPE OF TEST :
TDLo - Lowest published toxic dose
ROUTE OF EXPOSURE :
Subcutaneous
SPECIES OBSERVED :
Rodent - mouse
DOSE/DURATION :
1152 mg/kg/2Y-I
TOXIC EFFECTS :
Tumorigenic - equivocal tumorigenic agent by RTECS criteria Liver - tumors Reproductive - Tumorigenic effects - uterine tumors
TYPE OF TEST :
TD - Toxic dose (other than lowest)
ROUTE OF EXPOSURE :
Oral
SPECIES OBSERVED :
Rodent - rat
DOSE/DURATION :
11 gm/kg/51W-I
TOXIC EFFECTS :
Tumorigenic - Carcinogenic by RTECS criteria Sense Organs and Special Senses (Ear) - effect, not otherwise specified Skin and Appendages - tumors
TYPE OF TEST :
TD - Toxic dose (other than lowest)
ROUTE OF EXPOSURE :
Oral
SPECIES OBSERVED :
Rodent - rat
DOSE/DURATION :
6497 mg/kg/91W-C
TOXIC EFFECTS :
Tumorigenic - Carcinogenic by RTECS criteria Gastrointestinal - tumors Liver - tumors

MUTATION DATA

TYPE OF TEST :
Sister chromatid exchange
TEST SYSTEM :
Rodent - hamster Ovary
REFERENCE :
NTPTR* National Toxicology Program Technical Report Series. (Research Triangle Park, NC 27709) No.206- Volume(issue)/page/year: NTP-TR-372,1990 *** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA *** NIOSH RECOMMENDED EXPOSURE LEVEL (REL) : NIOSH REL TO o-DIANISIDINE-BASED DYES-air:CA lowest feasible conc. REFERENCE : NIOSH* National Institute for Occupational Safety and Health, U.S. Dept. of Health, Education, and Welfare, Reports and Memoranda. Volume(issue)/page/year: DHHS #92-100,1992 NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA : NOES - National Occupational Exposure Survey (1983) NOES Hazard Code - X7012 No. of Facilities: 7 (estimated) No. of Industries: 1 No. of Occupations: 1 No. of Employees: 489 (estimated) No. of Female Employees: 300 (estimated)
Symbol GHS07 GHS08
GHS07, GHS08
Signal Word Danger
Hazard Statements H302-H350
Precautionary Statements P201-P308 + P313
Personal Protective Equipment Eyeshields;full-face particle respirator type N100 (US);Gloves;respirator cartridge type N100 (US);type P1 (EN143) respirator filter;type P3 (EN 143) respirator cartridges
Hazard Codes T:Toxic
Risk Phrases R22;R45
Safety Phrases S53-S45
RIDADR 2811
WGK Germany 3
RTECS DD1050000
Packaging Group II
Hazard Class 6.1(a)
HS Code 2923900090
Precursor  0

DownStream  1

HS Code 2923900090
Summary 2923900090 other quaternary ammonium salts and hydroxides。Supervision conditions:None。VAT:17.0%。Tax rebate rate:9.0%。MFN tariff:6.5%。General tariff:30.0%
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