1352750-34-5
1352750-34-5 structure
- Name: WSP-1
- Chemical Name: wsp-1
- CAS Number: 1352750-34-5
- Molecular Formula: C33H21NO6S2
- Molecular Weight: 591.653
- Catalog: Research Areas Others
- Create Date: 2017-11-27 08:43:27
- Modify Date: 2025-08-25 11:42:02
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WSP-1 is a selective and rapid-reaction H2S specific fluorescent dye (Ex/Em=465/515 nm). WSP-1 reacts with H2S with the releasing of fluorophore[1].
| Name | wsp-1 |
|---|---|
| Synonyms |
6'-Methoxy-3-oxo-3H-spiro[2-benzofuran-1,9'-xanthen]-3'-yl 2-(2-pyridinyldisulfanyl)benzoate
Benzoic acid, 2-(2-pyridinyldithio)-, 6'-methoxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3'-yl ester |
| Description | WSP-1 is a selective and rapid-reaction H2S specific fluorescent dye (Ex/Em=465/515 nm). WSP-1 reacts with H2S with the releasing of fluorophore[1]. |
|---|---|
| Related Catalog | |
| In Vitro | WSP-1 具有荧光特性，激发波长 (ex) 465 nm，发射波长 (em) 515 nm[1]。 Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). 细胞内 H2S 的荧光检测 (荧光显微镜检查)[1]: 1. HASMCs 培养至约 90% 聚集，实验前 24 小时，将细胞以 30*103 每孔的密度在 8 孔培养载玻片上培养 24 小时。 2. 更换培养基，加入 WSP-1 工作液 400 μL，37℃ 孵育 30min。 3. 将 WSP-1 工作液替换为 380 μL 标准缓冲液，加入 20 μL 所需浓度的H2S 供体化合物。 4. 当 WSP-1 与 H2S 反应时，释放在 Ex/Em=465/515 nm 下可检测到的荧光团。取缓冲标准品，培养 1 小时后用 400 μL DPBS 洗涤。 5. 去除 DPBS，室温下每孔加入 200 μL Bouin 溶液固定细胞 10 min。去除多余的Bouin溶液，用 400 μL DPBS 清洗细胞 2 次。 6. 用荧光显微镜检查和获取荧光信号。 |
| References |
| Density | 1.5±0.1 g/cm3 |
|---|---|
| Boiling Point | 769.9±60.0 °C at 760 mmHg |
| Molecular Formula | C33H21NO6S2 |
| Molecular Weight | 591.653 |
| Flash Point | 419.4±32.9 °C |
| Exact Mass | 591.081055 |
| PSA | 134.55000 |
| LogP | 6.14 |
| Vapour Pressure | 0.0±2.6 mmHg at 25°C |
| Index of Refraction | 1.762 |
| Hazard Codes | Xn |
|---|
| Precursor 3 | |
|---|---|
| DownStream 0 | |
