Shanghai Jizhi Biochemical Technology Co., Ltd
China
Product Name: Acid Red 52
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Purity: 98.0%
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Dayang Chem (Hangzhou) Co., Ltd.
China
Product Name: Sulforhodamine B
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Zhejiang Hangyu API Co., Ltd
China
Product Name: SULFORHODAMINE B
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Henan Tianfu Chemical Co., Ltd.
China
Product Name: SULFORHODAMINE B
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3520-42-1

3520-42-1 structure

Name: Acid Red 52
Chemical Name: lissamine rhodamine
CAS Number: 3520-42-1
Molecular Formula: C₂₇H₂₉N₂NaO₇S₂
Molecular Weight: 580.65
Catalog: Dyes and pigments dye Acid Dyestuff
Create Date: 2018-09-01 13:18:01
Modify Date: 2024-01-02 08:41:30
Sulforhodamine B sodium salt is a fluorescent dye with uses spanning from laser-induced fluorescence (LIF) to the quantification of cellular proteins of cultured cells.

Name	lissamine rhodamine
Synonyms	4'-Butoxy-3-piperidinopropiophenone hydrochloride Tanaclone EINECS 222-529-8 dyclonine hydrochloride SULFORHODAMINE B MFCD00010180 C.I. 45100 Xanthylium, 3,6-bis(diethylamino)-9-(2,4-disulfophenyl)-, inner salt, sodium salt (1:1) dye acid red 52 Sodium 4-[6-(diethylamino)-3-(diethyliminio)-3H-xanthen-9-yl]benzene-1,3-disulfonate Sulforhodamine-B Dyclone N,N,N',N'-tetraethylsulforhodamine rhodamine 200 S sodium salt Dyclocaine hydrochloride Dyclonine HCL Acid Red 52,Sulforhodamine B monosodium salt Dyclothane Acid Red 52 C.I.Acid Red 52 Sulforhodamine B sodium salt Sulforhodamine B (sodium salt)

Description	Sulforhodamine B sodium salt is a fluorescent dye with uses spanning from laser-induced fluorescence (LIF) to the quantification of cellular proteins of cultured cells.
Related Catalog	Signaling Pathways >> Others >> Others Dye Reagents Research Areas >> Others
In Vitro	Sulforhodamine B (SRB) is often used as a membrane-impermeable polar tracer or used for cell density determination via determination of cellular proteins (cytotoxicity assay). The SRB assay has been used to inexpensively conduct various screening assays to investigate cytotoxicity in cell based studies. This method relies on the property of SRB, which binds stoichiometrically to proteins under mild acidic conditions and then can be extracted using basic conditions; thus, the amount of bound dye can be used as a proxy for cell mass, which can then be extrapolated to measure cell proliferation. The protocol can be divided into four main steps: preparation of treatment, incubation of cells with treatment of choice, cell fixation and SRB staining, and absorbance measurement. This assay is limited to manual or semiautomatic screening, and can be used in an efficient and sensitive manner to test chemotherapeutic drugs or small molecules in adherent cells. It also has applications in evaluating the effects of gene expression modulation (knockdown, gene expression upregulation), as well as to study the effects of miRNA replacement on cell proliferation[1].
Cell Assay	Gently add 25 μL (96-well format) or 5 μL (384-well format) cold 50% (wt/vol) TCA to each well directly to medium supernatant, and incubate the plates at 4 °C for 1 h. Mixing is not required, as this could lead to some cells detaching from the bottom of the well. Wash the plates four times by submerging the plate in a tub with slow-running tap water and remove excess water by gently tapping the plate into a paper towel. After the last wash allow the plate to air-dry at room temperature. Add 50 μL (96-well format) or 20 μL (384-well format) of 0.04% (wt/vol) SRB solution to each well. Leave at room temperature for 1 h and then quickly rinse the plates four times with 1% (vol/vol) acetic acid (200 μL for 96-well format or 30 μL for 384-well format) to remove unbound dye. Allow the plate to air-dry at room temperature[1].
References	[1]. Orellana EA, et al. Sulforhodamine B (SRB) Assay in Cell Culture to Investigate Cell Proliferation. Bio Protoc. 2016 Nov 5;6(21). pii: e1984.

Molecular Formula	C₂₇H₂₉N₂NaO₇S₂
Molecular Weight	580.65
PSA	150.78000
LogP	7.81310
Water Solubility	methanol: 1 mg/mL

CHEMICAL IDENTIFICATION

RTECS NUMBER :: BP6750000

CHEMICAL NAME :: Ammonium, (6-(diethylamino)-9-(2,4-disulfophenyl)-3H-xanthen-3- ylidene)diethyl-, hydroxide, inner salt, sodium salt

CAS REGISTRY NUMBER :: 3520-42-1

LAST UPDATED :: 199701

DATA ITEMS CITED :: 5

MOLECULAR FORMULA :: C27-H29-N2-O7-S2.Na

MOLECULAR WEIGHT :: 580.69

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Oral

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 10300 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

MUTATION DATA

TYPE OF TEST :: Cytogenetic analysis

TEST SYSTEM :: Rodent - hamster Fibroblast

DOSE/DURATION :: 13100 mg/L

REFERENCE :: FCTOD7 Food and Chemical Toxicology. (Pergamon Press Inc., Maxwell House, Fairview Park, Elmsford, NY 10523) V.20- 1982- Volume(issue)/page/year: 22,623,1984 *** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA *** NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA : NOHS - National Occupational Hazard Survey (1974) NOHS Hazard Code - M2208 No. of Facilities: 114 (estimated) No. of Industries: 5 No. of Occupations: 7 No. of Employees: 901 (estimated) NOES - National Occupational Exposure Survey (1983) NOES Hazard Code - M2208 No. of Facilities: 2248 (estimated) No. of Industries: 47 No. of Occupations: 51 No. of Employees: 75084 (estimated) No. of Female Employees: 12598 (estimated)

Symbol	GHS07
Signal Word	Warning
Hazard Statements	H315-H319-H335
Precautionary Statements	P261-P305 + P351 + P338
Personal Protective Equipment	Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
Hazard Codes	Xi
Risk Phrases	36/37/38
Safety Phrases	22-24/25
RIDADR	NONH for all modes of transport
WGK Germany	2
RTECS	BP6750000
HS Code	3204120000

Precursor 0
DownStream 1
62796-29-6

HS Code	2921499090
Summary	2921499090 other aromatic monoamines and their derivatives; salts thereof VAT:17.0% Tax rebate rate:9.0% Supervision conditions:none MFN tariff:6.5% General tariff:30.0%

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