926908-04-5
926908-04-5 structure
- Name: HNHA
- Chemical Name: N-hydroxy-7-naphthalen-2-ylsulfanylheptanamide
- CAS Number: 926908-04-5
- Molecular Formula: C17H21NO2S
- Molecular Weight: 303.42
- Catalog: Signaling Pathways Cell Cycle/DNA Damage HDAC
- Create Date: 2016-11-26 09:02:22
- Modify Date: 2025-09-01 10:37:50
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HNHA is a potent histone deacetylase (HDAC) inhibitor. HNHA arrests the cell cycle at the G1/S phase via p21 induction. HNHA inhibits tumor growth and tumor neovascularization. HNHA may be a potent anti-cancer agent against breast cancer[1].
| Name | N-hydroxy-7-naphthalen-2-ylsulfanylheptanamide |
|---|---|
| Synonyms |
N-hydroxy-7-(2-naphthylthio)heptanamide
N-Hydroxy-7-(2-naphthylsulfanyl)heptanamide Heptanamide, N-hydroxy-7-(2-naphthalenylthio)- hnha |
| Description | HNHA is a potent histone deacetylase (HDAC) inhibitor. HNHA arrests the cell cycle at the G1/S phase via p21 induction. HNHA inhibits tumor growth and tumor neovascularization. HNHA may be a potent anti-cancer agent against breast cancer[1]. |
|---|---|
| Related Catalog | |
| Target |
MMP-2 MMP-9 |
| In Vitro | HNHA (0-100 μM, 96 h) shows strong inhibition at lower concentrations on cancer cell lines, especially on breast cancer cells, mouse FM3A and human MCF-7[1]. HNHA (15 μM, 24 h) arrests cancer cells at the G1/S phase of the cell cycle, activates p21and rescues strongly protein acetylation[1]. HNHA (15 μM, 12 h) inhibits angiogenic proteins in breast cancer cells, effectively inactivates MMP-2, MMP-9, VEGF and HIF-1α[1]. Cell Proliferation Assay Cell Line: FM3A, C1300, LA-N-1, LA-N-2, LA-N-5, NB16, NB19, NB69, SK-N-SH, MCF-7 and HT-29[1] Concentration: 0-100 μM Incubation Time: 96 h Result: Showed strong inhibition at lower concentrations on all cancer cell lines (FM3A, C1300, LA-N-1, LA-N-2, LA-N-5, NB16, NB19, NB69, SK-N-SH, MCF-7 and HT-29), with IC50 values of 15.70, 55.63, 22.78, 23.18, 26.70, 19.64, 21.26, 22.31, 65.09, 14.33, and 16.98 μM, respectively. Cell Viability Assay Cell Line: FM3A and MCF-7[1] Concentration: 0, 0.1, 1, 5, 10, 15, 20, 25,30 μM Incubation Time: 48 h Result: Showed dose-dependent inhibition of viability in mouse and human breast cancer cells. Cell Cycle Analysis Cell Line: FM3A and MCF-7 cells[1] Concentration: 15 μM Incubation Time: 24 h Result: Arrested FM3A and MCF-7 cells in the G1/S phase. Western Blot Analysis Cell Line: FM3A and MCF-7 cells[1] Concentration: 0, 0.1, 1, 10, and 20 μM (24 h) Incubation Time: 1, 6, 24, 48, and 72 h (15 μM) Result: Activated a cell proliferation arrestor p21, increased histone and non-histone protein acetylation and inhibited FM3A and MCF-7 proliferation in vitro, and was very effective in increasing the acetylation level of histone H3 protein in FM3A and MCF-7. The most effective dose point for acetylation of histone H3 was 10-20 μM. Histone H3 acetylation peaked after 1 h of exposure to the drugs and remained stable for 1-6 h. Western Blot Analysis Cell Line: FM3A and MCF-7 cells[1] Concentration: 15 μM Incubation Time: 12 h Result: Showed a strong induction of TIMP-1 and TIMP-2, and effectively inactivated MMP-2, MMP-9, VEGF and HIF-1α. |
| In Vivo | HNHA (20 μM/mouse, IP, once every 2 days for a total of six injections) reduces tumor burden and extends the survival rate, activates TIMP-1, TIMP-2 and p21 and inhibits MMP-2, MMP-9, HIF-1α and VEGF protein expression[1]. Animal Model: C3H/HeJ-FasL mice (FM3A breast cancer cell tumor xenograft, 6 weeks, n = 25/group)[1] Dosage: 20 μM/mouse Administration: IP, once every 2 days for a total of six injections Result: Reduced tumor burden and extended the survival rate. Effectively inhibited cancer development and angiogenesis in vivo. Increased TIMP-1, TIMP-2 and p21, decreased MMP-2, MMP-9, HIF-1α and VEGF protein expression, and reduced the distribution of CD34, HIF-1α and VEGF. |
| References |
| Density | 1.2±0.1 g/cm3 |
|---|---|
| Molecular Formula | C17H21NO2S |
| Molecular Weight | 303.42 |
| Exact Mass | 303.129303 |
| PSA | 74.63000 |
| LogP | 3.71 |
| Index of Refraction | 1.625 |
| Storage condition | 2-8°C |
| Hazard Codes | Xi |
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