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553-24-2

553-24-2 structure
553-24-2 structure
  • Name: Neutral Red
  • Chemical Name: neutral red
  • CAS Number: 553-24-2
  • Molecular Formula: C15H17ClN4
  • Molecular Weight: 288.775
  • Catalog: Dyes and pigments dye Neutral dye
  • Create Date: 2018-04-16 08:00:00
  • Modify Date: 2025-08-21 09:58:02
  • Neutral Red, a nitrogenous pH-indicator with a pKi of 6.8, is an indicator for the internal acidification of thylakoids. Neutral Red stains lysosomes red[1].
Name neutral red
Synonyms N2,N2,7-Trimethylphenazine-2,8-diamine hydrochloride
3-amino-7-dimethylamino-2-methyl-phenazinium chloride
Nuclear Fast Red
NEUTRAL RED
Neutral Red W
Toluylene Red
Neutral Red chloride
3-Amino-7-dimethylamino-2-methylphenazine hydrochloride
2,8-Phenazinediamine, N8,N8,3-trimethyl-, monohydrochloride
Michrome No. 226
Neutral Red (Tautomer)
Basic Red 5
EINECS 209-035-8
8-N,8-N,3-trimethylphenazine-2,8-diamine,hydrochloride
Neutral Red Base
N8,N8,3-trimethyl-2,8-phenazinediamine monohydrochloride
C.I. Basic Red 5
N,N,3-Trimethylphenazine-2,8-diamine hydrochloride (1:1)
2,8-Phenazinediamine, N,N,3-trimethyl-, hydrochloride (1:1)
N,N,3-Trimethyl-2,8-phenazinediamine hydrochloride (1:1)
N8,N8,3-trimethylphenazine-2,8-diamine hydrochloride
MFCD04119237
Description Neutral Red, a nitrogenous pH-indicator with a pKi of 6.8, is an indicator for the internal acidification of thylakoids. Neutral Red stains lysosomes red[1].
Related Catalog
In Vitro As cells begin to die, their ability to incorporate neutral red diminishes. The neutral red is also used to stain cell cultures for plate titration of viruses[2].
References

[1]. Winfried AUSLANDER, et al. NEUTRAL RED, A RAPID INDICATOR FOR pH-CHANGES IN THE INNER PHASE OF THYLAKOIDS. FEBS LETTERS. 1975 Nov.

[2]. Ellen Borenfreund, et al. A simple quantitative procedure using monolayer cultures for cytotoxicity assays (HTD/NR-90). Journal of tissue culture methods volume 9, pages7–9 (1985).
Boiling Point 497.3ºC at 760mmHg
Melting Point 290 °C (dec.)(lit.)
Molecular Formula C15H17ClN4
Molecular Weight 288.775
Exact Mass 288.114166
PSA 55.04000
LogP 4.12280
Water Solubility 50 g/L

CHEMICAL IDENTIFICATION

RTECS NUMBER :
SG1400000
CHEMICAL NAME :
Phenazine, 3-amino-7-(dimethylamino)-2-methyl-, hydrochloride
CAS REGISTRY NUMBER :
553-24-2
LAST UPDATED :
199701
DATA ITEMS CITED :
22
MOLECULAR FORMULA :
C15-H16-N4.Cl-H
MOLECULAR WEIGHT :
288.81
WISWESSER LINE NOTATION :
T C666 BN INJ E1 FZ LN1&1 &GH

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

TYPE OF TEST :
LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE :
Intravenous
SPECIES OBSERVED :
Rodent - rat
DOSE/DURATION :
112 mg/kg
TOXIC EFFECTS :
Behavioral - convulsions or effect on seizure threshold Cardiac - other changes Lungs, Thorax, or Respiration - other changes
TYPE OF TEST :
LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE :
Intraperitoneal
SPECIES OBSERVED :
Rodent - mouse
DOSE/DURATION :
432 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
TYPE OF TEST :
LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE :
Intravenous
SPECIES OBSERVED :
Rodent - mouse
DOSE/DURATION :
142 mg/kg
TOXIC EFFECTS :
Behavioral - convulsions or effect on seizure threshold Cardiac - other changes Lungs, Thorax, or Respiration - other changes
TYPE OF TEST :
LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE :
Intravenous
SPECIES OBSERVED :
Rodent - rabbit
DOSE/DURATION :
96600 ug/kg
TOXIC EFFECTS :
Behavioral - convulsions or effect on seizure threshold Lungs, Thorax, or Respiration - respiratory stimulation Lungs, Thorax, or Respiration - other changes
TYPE OF TEST :
Sex chromosome loss and nondisjunction
TYPE OF TEST :
Sister chromatid exchange
TYPE OF TEST :
DNA damage
TYPE OF TEST :
Cytogenetic analysis

MUTATION DATA

TEST SYSTEM :
Bird - chicken
DOSE/DURATION :
85 ug/kg
REFERENCE :
47JMAE "Cytogenetic Assays of Environmental Mutagens," Hsu, T.C., ed., Totowa, NJ, Allanheld, Osmun & Co., 1982 Volume(issue)/page/year: -,137,1982 *** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA *** NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA : NOHS - National Occupational Hazard Survey (1974) NOHS Hazard Code - M1598 No. of Facilities: 420 (estimated) No. of Industries: 5 No. of Occupations: 5 No. of Employees: 694 (estimated) NOES - National Occupational Exposure Survey (1983) NOES Hazard Code - M1598 No. of Facilities: 746 (estimated) No. of Industries: 13 No. of Occupations: 12 No. of Employees: 23740 (estimated) No. of Female Employees: 19202 (estimated)
Personal Protective Equipment Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
Hazard Codes Xn:Harmful;
Risk Phrases R68
Safety Phrases S36/37
RIDADR NONH for all modes of transport
WGK Germany 3
RTECS SG1400000
HS Code 32041300
HS Code 2933990090
Summary 2933990090. heterocyclic compounds with nitrogen hetero-atom(s) only. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0%
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