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1239-45-8

1239-45-8 structure
1239-45-8 structure
  • Name: Ethidium bromide
  • Chemical Name: ethidium bromide
  • CAS Number: 1239-45-8
  • Molecular Formula: C21H20BrN3
  • Molecular Weight: 394.308
  • Catalog: Biochemical Biochemical reagent Biological dye
  • Create Date: 2018-08-30 15:24:22
  • Modify Date: 2024-01-02 12:30:30
  • Ethidium bromide is an intercalating agent commonly used as a fluorescent tag (nucleic acid stain) in molecular biology laboratories for techniques such as agarose gel electrophoresis.
Name ethidium bromide
Synonyms 3,8-Diamino-5
MFCD00011724
3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide,EtBr,Homidium bromide
Phenanthridinium, 3,8-diamino-5-ethyl-6-phenyl-, bromide (1:1)
Homidium bromide
3,8-Diamino-5-ethyl-6-phenylphenanthridin-5-ium bromide
3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide
EINECS 214-984-6
5-ethyl-6-phenylphenanthridin-5-ium-3,8-diamine,bromide
EthidiuM BroMide
Description Ethidium bromide is an intercalating agent commonly used as a fluorescent tag (nucleic acid stain) in molecular biology laboratories for techniques such as agarose gel electrophoresis.
Related Catalog
In Vitro Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). The preparation of agarose gel: 1． Agarose gels are commonly used in concentrations of 0.5% to 2.5% depending on the size of bands needed to be separated. 2． Mix the agarose powder with 1X TAE/TBE. 3． Microwave for 1-3 min until the agarose is completely dissolved (Caution: not overboil). 4． Make the solution cool down before solidification. 5． Add ethidium bromide (EtBr) to a final concentration of approximately 0.2-0.5 μg/mL (Stocks are generally 10 mg/ml, and require 5 µL stock/100 mL gel). 6． Ethidium bromide binds to the DNA and you could visualize the DNA under ultraviolet (UV) light. CAUTION: EtBr is a known mutagen. Please pay attention to strengthening protection.
References

[1]. P Stevenson, et al. Comparison of isometamidium chloride and homidium bromide as prophylactic drugs for trypanosomiasis in cattle at Nguruman, Kenya. Acta Trop. 1995 May;59(2):77-84.

[2]. Sabnis, Ram Wasudeo (2010). Handbook of Biological Dyes and Stains: Synthesis and Industrial Application. Hoboken, NJ: Wiley. ISBN 978-0-470-40753-0.
Melting Point 260-262 °C (dec.)(lit.)
Molecular Formula C21H20BrN3
Molecular Weight 394.308
Flash Point >100°C
Exact Mass 393.084045
PSA 55.92000
LogP 2.29820
Index of Refraction 1.4575 (20ºC)
Storage condition 2-8°C
Stability Stable. Incompatible with strong oxidizing agents.
Water Solubility 40 g/L (25 ºC)

CHEMICAL IDENTIFICATION

RTECS NUMBER :
SF7950000
CHEMICAL NAME :
Phenanthridinium, 3,8-diamino-5-ethyl-6-phenyl-, bromide
CAS REGISTRY NUMBER :
1239-45-8
LAST UPDATED :
199712
DATA ITEMS CITED :
79
MOLECULAR FORMULA :
C21-H20-N3.Br
MOLECULAR WEIGHT :
394.35
WISWESSER LINE NOTATION :
T B666 HKJ EJ H2 IR& LZ &E &9/26

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

TYPE OF TEST :
LDLo - Lowest published lethal dose
ROUTE OF EXPOSURE :
Intraperitoneal
SPECIES OBSERVED :
Rodent - mouse
DOSE/DURATION :
20 mg/kg
TOXIC EFFECTS :
Tumorigenic - active as anti-cancer agent
TYPE OF TEST :
LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE :
Subcutaneous
SPECIES OBSERVED :
Rodent - mouse
DOSE/DURATION :
110 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
TYPE OF TEST :
Sex chromosome loss and nondisjunction
TYPE OF TEST :
Specific locus test
TYPE OF TEST :
Dominant lethal test
TYPE OF TEST :
Micronucleus test
TYPE OF TEST :
DNA inhibition
TYPE OF TEST :
Sister chromatid exchange

MUTATION DATA

TEST SYSTEM :
Bird - chicken
DOSE/DURATION :
847 ug/kg
REFERENCE :
47JMAE "Cytogenetic Assays of Environmental Mutagens," Hsu, T.C., ed., Totowa, NJ, Allanheld, Osmun & Co., 1982 Volume(issue)/page/year: -,137,1982 *** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA *** NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA : NOHS - National Occupational Hazard Survey (1974) NOHS Hazard Code - A1168 No. of Facilities: 17 (estimated) No. of Industries: 1 No. of Occupations: 1 No. of Employees: 34 (estimated) NOES - National Occupational Exposure Survey (1983) NOES Hazard Code - A1168 No. of Facilities: 7 (estimated) No. of Industries: 1 No. of Occupations: 3 No. of Employees: 21 (estimated)
Symbol GHS06 GHS08
GHS06, GHS08
Signal Word Danger
Hazard Statements H302-H330-H341
Precautionary Statements P260-P281-P284-P310
Personal Protective Equipment Eyeshields;Faceshields;full-face particle respirator type N100 (US);Gloves;respirator cartridge type N100 (US);type P1 (EN143) respirator filter;type P3 (EN 143) respirator cartridges
Hazard Codes T+:Verytoxic;
Risk Phrases R21/22;R26;R36/37/38;R68
Safety Phrases S36/37-S45-S36/37/39-S28A-S26-S22
RIDADR UN 2811 6.1/PG 1
WGK Germany 3
RTECS SF7950000
Packaging Group I
Hazard Class 6.1
HS Code 29339990
HS Code 2933990090
Summary 2933990090. heterocyclic compounds with nitrogen hetero-atom(s) only. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0%

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