634175-34-1

634175-34-1 structure

Name: ML328
Chemical Name: 8-Ethyl-5-oxo-2-(4-{[3-(trifluoromethyl)phenyl]carbamothioyl}-1-piperazinyl)-5,8-dihydropyrido[2,3-d]pyrimidine-6-carboxylic acid
CAS Number: 634175-34-1
Molecular Formula: C₂₂H₂₁F₃N₆O₃S
Molecular Weight: 506.501
Catalog: Signaling Pathways Anti-infection Bacterial
Create Date: 2021-06-01 14:09:03
Modify Date: 2024-01-08 20:50:40
ML328 is a selective inhibitor of bacterial AddAB and RecBCD helicase-nucleases with IC50 values of 26 and 5.1 μM, respectively. ML328 is a gyrase inhibitor. ML328 strongly inhibits the growth of E. coli in the presence of phage. ML328 can be used for the research of bacterial infection[1][2].

Name	8-Ethyl-5-oxo-2-(4-{[3-(trifluoromethyl)phenyl]carbamothioyl}-1-piperazinyl)-5,8-dihydropyrido[2,3-d]pyrimidine-6-carboxylic acid
Synonyms	8-Ethyl-5-oxo-2-(4-{[3-(trifluoromethyl)phenyl]carbamothioyl}-1-piperazinyl)-5,8-dihydropyrido[2,3-d]pyrimidine-6-carboxylic acid Pyrido[2,3-d]pyrimidine-6-carboxylic acid, 8-ethyl-5,8-dihydro-5-oxo-2-[4-[thioxo[[3-(trifluoromethyl)phenyl]amino]methyl]-1-piperazinyl]-

Description	ML328 is a selective inhibitor of bacterial AddAB and RecBCD helicase-nucleases with IC50 values of 26 and 5.1 μM, respectively. ML328 is a gyrase inhibitor. ML328 strongly inhibits the growth of E. coli in the presence of phage. ML328 can be used for the research of bacterial infection[1][2].
Related Catalog	Research Areas >> Infection Signaling Pathways >> Anti-infection >> Bacterial
In Vitro	ML328 (0.1-1000 μM) 具有 AddAB 和 RecBCD 核酸酶抑制活性，IC50 值分别为 26 和 5.1 μM [1]。 ML328 对 RecBCD，RecF 和 RecE 通路特异性地抑制高频重组[1]。 ML328 对 RecBCD 具有抑制作用，对 RecBCD 核酸酶、促进 Hfr 重组和噬菌体 λ 重组的 IC50 值分别为 3，0.3 和 5 μΜ[2]。 ML328 抑制 E. coli RecBCD，E. coli AddAB，M. smeg AddAB 和 M. smeg RecBCD 的 IC50 值分别为 4.6，16，2.4 和 5.5 μM[2]。 Ml328 (25 μΜ；2 h) 降低了 H2O2 诱导 E. coli 突变的频率[2]。 Ml328 (25 μΜ；1 h) 降低 H2O2 诱导 E. coli 缬氨酸抗性 (valineR) 突变的频率[2]。 ML328 (50 μΜ) 轻度抑制 E. coli 生长，但在噬菌体存在时显著抑制 E. coli 生长[2]。
References	[1]. Amundsen SK, et al. Small-molecule inhibitors of bacterial AddAB and RecBCD helicase-nuclease DNA repair enzymes. ACS Chem Biol. 2012 May 18;7(5):879-91. [2]. SMITH GERALD R, et al. ANTIBIOTIC COMPOUNDS AND COMPOSITIONS, AND METHODS FOR IDENTIFICATION THEREOF. WO/2013/142628. 2014.