Nanjing Peptide Biotech Ltd
China
Product Name: Boc-Lys(Ac)-AMC
Price: ￥Inquiry/1kg ￥Inquiry/1g ￥Inquiry/1mg ￥Inquiry/1ton
Purity: 98.0%
Stocking Period: Inquiry
Contact: jom

Zhejiang Hangyu API Co., Ltd
China
Product Name: Boc-Lys(Ac)-AMC
Price: $Inquiry/1kg
Purity: 99.0%
Stocking Period: 1 Day
Contact: Vinnie

233691-67-3

233691-67-3 structure

233691-67-3 structure

Name: Boc-Lys(Ac)-AMC
Chemical Name: tert-butyl N-[(2S)-6-acetamido-1-[(4-methyl-2-oxochromen-7-yl)amino]-1-oxohexan-2-yl]carbamate
CAS Number: 233691-67-3
Molecular Formula: C₂₃H₃₁N₃O₆
Molecular Weight: 445.50900
Catalog: Signaling Pathways Cell Cycle/DNA Damage HDAC
Create Date: 2017-11-09 16:16:36
Modify Date: 2024-01-17 16:28:22
Boc-Lys(Ac)-AMC is a cell-permeable fluorometric HDAC substrate (Ex/Em = 355 nm/460 nm)[1][2].

Name	tert-butyl N-[(2S)-6-acetamido-1-[(4-methyl-2-oxochromen-7-yl)amino]-1-oxohexan-2-yl]carbamate
Synonyms	(S)-tert-Butyl (6-acetamido-1-((4-methyl-2-oxo-2H-chromen-7-yl)amino)-1-oxohexan-2-yl)carbamate Histone Deacetylase Substrate,Fluorogenic Nalpha-Boc-Nepsilon-acetyl-L-lysine 7-amido-4-methylcoumarin N-(4-Methyl-7-coumarinyl)-Nalpha-(t-butoxycarbonyl)-Nomega-acetyllysinamide Boc-Ac-Lys-AMC Boc-Lys(Ac)-AMC Boc-L(Ac)-AMC Boc-Lys(Ac)-7-Amino-4-Methylcoumarin tert-Boc (N-acetyl-Lys)-AMC

Description	Boc-Lys(Ac)-AMC is a cell-permeable fluorometric HDAC substrate (Ex/Em = 355 nm/460 nm)[1][2].
Related Catalog	Research Areas >> Others Signaling Pathways >> Cell Cycle/DNA Damage >> HDAC Signaling Pathways >> Epigenetics >> HDAC
In Vitro	Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). 基于组织培养的 HDAC 检测[2] 1. 将 6 × 104 细胞在 100 mL 培养基中加入 25 μM Boc-Lys(Ac)-AMC 于 96 孔组织培养板中培养过夜。 2. 37°C，5% CO2 下孵育 2-3 小时。 3. 加入含有细胞裂解缓冲液和胰蛋白酶试剂的 HADC 显影液，终止脱乙酰反应。 4. 室温孵育 15 分钟。 5. 在 Ex/Em = 355 nm/460 nm 处，用 SpectraMax M5 微板仪测量 AMC 的荧光信号。
References	[1]. Reddy DS, et al. Measuring Histone Deacetylase Inhibition in the Brain. Curr Protoc Pharmacol. 2018 Jun;81(1):e41. [2]. Ueki N, et al. Selective cancer targeting with prodrugs activated by histone deacetylases and a tumour-associated protease. Nat Commun. 2013;4:2735.

Molecular Formula	C₂₃H₃₁N₃O₆
Molecular Weight	445.50900
Exact Mass	445.22100
PSA	126.74000
LogP	4.09440

Hazard Codes	Xi

26093-31-2 structure

26093-31-2

6404-26-8 structure

6404-26-8

~38%

233691-67-3 structure

233691-67-3

Literature: Hoffmann; Brosch; Loidl; Jung Pharmazie, 2000 , vol. 55, # 8 p. 601 - 606

Precursor 2
26093-31-2 6404-26-8
DownStream 1
26093-31-2