161180-11-6

161180-11-6 structure

Name: 7-Hydroxycoumarinyl arachidonate
Chemical Name: (2-oxochromen-7-yl) icosa-5,8,11,14-tetraenoate
CAS Number: 161180-11-6
Molecular Formula: C₂₉H₃₆O₄
Molecular Weight: 448.594
Catalog: Signaling Pathways Metabolic Enzyme/Protease MAGL
Create Date: 2016-11-30 14:17:19
Modify Date: 2025-08-25 13:44:10
7-Hydroxycoumarinyl arachidonate (7-HCA) is a fluorogenic substrate of cytosolic phospholipase A2 (PLA2). 7-Hydroxycoumarinyl arachidonate is also a fluorogenic substrate for monoacylglycerol lipase (MAGL). MAGL protein catalyzes the hydrolysis of 7-Hydroxycoumarinyl arachidonat to generate Arachidonic acid (AA) and the highly fluorescent 7-hydroxyl coumarin (7-HC; HY-N0573). Release of 7-HC can be measured using a fluorometer[1][2][3].

Name	(2-oxochromen-7-yl) icosa-5,8,11,14-tetraenoate
Synonyms	7-HC-arachidonate Umbelliferyl Arachidonate 2-Oxo-2H-chromen-7-yl (5Z,8Z,11Z,14Z)-5,8,11,14-icosatetraenoate 5,8,11,14-Eicosatetraenoic acid, 2-oxo-2H-1-benzopyran-7-yl ester, (5Z,8Z,11Z,14Z)-

Description	7-Hydroxycoumarinyl arachidonate (7-HCA) is a fluorogenic substrate of cytosolic phospholipase A2 (PLA2). 7-Hydroxycoumarinyl arachidonate is also a fluorogenic substrate for monoacylglycerol lipase (MAGL). MAGL protein catalyzes the hydrolysis of 7-Hydroxycoumarinyl arachidonat to generate Arachidonic acid (AA) and the highly fluorescent 7-hydroxyl coumarin (7-HC; HY-N0573). Release of 7-HC can be measured using a fluorometer[1][2][3].
Related Catalog	Signaling Pathways >> Metabolic Enzyme/Protease >> Phospholipase Research Areas >> Others Signaling Pathways >> Metabolic Enzyme/Protease >> MAGL
In Vitro	MAGL catalyzes the hydrolysis of 7-Hydroxycoumarinyl arachidonate (7-HCA) into arachidonic acid (AA) and 7-HC. Release of 7-HC, which is fluorescent, is monitored over time with excitation (Ex) at 355 nm and emission (Em) at 460 nm[1].
References	[1]. Yuren Wang, et al. A fluorescence-based assay for monoacylglycerol lipase compatible with inhibitor screening. Assay Drug Dev Technol. 2008 Jun;6(3):387-93. [2]. Z Huang, et al. A continuous fluorescence-based assay for the human high-molecular-weight cytosolic phospholipase A2. Anal Biochem. 1994 Oct;222(1):110-5. [3]. R T Pickard, et al. Identification of essential residues for the catalytic function of 85-kDa cytosolic phospholipase A2. Probing the role of histidine, aspartic acid, cysteine, and arginine. J Biol Chem. 1996 Aug 9;271(32):19225-31.