DC Chemicals Limited
China
Product Name: Aftin-4
Price: $600.0/100mg $1100.0/250mg $2100.0/1g
Purity: 98.0%
Stocking Period: 3 Day
Contact: Tony Cao

866893-90-5

866893-90-5 structure

866893-90-5 structure

Name: Aftin-4
Chemical Name: 1-Butanol, 2-[[9-(1-methylethyl)-6-[methyl(phenylmethyl)amino]-9H-purin-2-yl]amino]-, (2R)
CAS Number: 866893-90-5
Molecular Formula: C₂₀H₂₈N₆O
Molecular Weight: 368.47600
Catalog: Signaling Pathways Neuronal Signaling Amyloid-β
Create Date: 2016-10-01 20:12:26
Modify Date: 2024-01-05 14:39:57
Aftin-4 is an Amyloid-β42 (Aβ42) inducer.

Name	1-Butanol, 2-[[9-(1-methylethyl)-6-[methyl(phenylmethyl)amino]-9H-purin-2-yl]amino]-, (2R)
Synonyms	Aftin 4 Aftin-4

Description	Aftin-4 is an Amyloid-β42 (Aβ42) inducer.
Related Catalog	Signaling Pathways >> Neuronal Signaling >> Amyloid-β Research Areas >> Neurological Disease
Target	Amyloid-β[1]
In Vitro	Aftin-4 selectively and potently increases Aβ1-42 in N2a cells, primary neurons, and brain lysates, with an EC50 value around 30 μM[1].
In Vivo	Aftin-4 increases Aβ1-42 levels in vivo in mice and provokes rapidly a sustained toxicity highly reminiscent of Alzheimer’s disease (AD). Aftin-4 is administered at increasing doses, between 3 and 20 nmol/mouse, into the lateral ventricle and animals are sacrificed at various time points, between 3 to 14 days after injection. The hippocampus is dissected out the contents in Aβ1-40 or Aβ1-42 is determined using a mouse ELISA assay. Aftin-4 dose-dependently and significantly increases Aβ1-42 content, up to +216% at the highest dose tested[1].
Animal Admin	Mice[1] Male Swiss OF-1 mice, aged 7-9 weeks and weighing 32±2 g are used. Aftin-4 is solubilized in DMSO at a concentration of 3 mg/mL and stored at -20°C until use. Aftin-4 is administered intracerebroventricularly (i.c.v. ), with a Hamilton microsyringe equipped with a 3-mm needle in a final volume of 3 μL per mouse. The injection coordinates are -0.4 mm with respect to bregma, 1.00mm to the right from the central, and 2.50mm in depth. Aftin-4 is also injected intraperitoneally (i.p.). Aftin-4 is solubilized in DMSO 40% in distilled water at 2, 6 or 20 mg/mL and administered in a final volume of 100 μL/20 g body weight[1].
References	[1]. Meunier J, et al. Brain toxicity and inflammation induced in vivo in mice by the amyloid-β forty-two inducer aftin-4, a roscovitine derivative. J Alzheimers Dis. 2015;44(2):507-24.

Molecular Formula	C₂₀H₂₈N₆O
Molecular Weight	368.47600
Exact Mass	368.23200
PSA	79.10000
LogP	3.29940