333338-18-4

333338-18-4 structure

Name: 4-Nitrophenyl phosphate disodium hexahydrate
Chemical Name: disodium,(4-nitrophenyl) phosphate,hexahydrate
CAS Number: 333338-18-4
Molecular Formula: C₆H₁₆NNa₂O₁₂P
Molecular Weight: 371.14400
Catalog: Chemical reagent Organic reagent Azo, diazonium compound
Create Date: 2019-01-01 18:52:48
Modify Date: 2025-08-25 09:49:58
4-Nitrophenyl phosphate (p-nitrophenyl phosphate) disodium hexahydrate is widely used as a small molecule phosphotyrosine-like substrate in activity assays for protein tyrosine phosphatases. 4-Nitrophenyl phosphate disodium hexahydrate is a colorless substrate that upon hydrolysis is converted to a yellow 4-nitrophenolate ion that can be monitored by absorbance at 405 nm[1].

Name	disodium,(4-nitrophenyl) phosphate,hexahydrate
Synonyms	MFCD00066288 di-Sodium 4-nitrophenyl phosphate pNPP disodium salt hexahydrate DisodiuM 4-Nitrophenylphosphate Hexahydrate Phosphoric Acid 4-Nitrophenyl Ester Disodium Salt Hexahydrate Phosphatase substrate 4-Nitrophenyl Phosphate,Disodium Salt Hexahydrate p-Nitrophenyl phosphate disodium hexahydrate pNPP disodium hexahydrate 4-Nitrophenyl phosphate disodium salt hexahydrate p-Nitrophenyl phosphate disodium hexahydrate,Disodium 4-nitrophenyl phosphate hexahydrate,di Sodium 4-nitrophenyl phosphate hexahydrate EINECS 224-246-5 Disodium 4-Nitrophenyl Phosphate Hexahydrate

Description	4-Nitrophenyl phosphate (p-nitrophenyl phosphate) disodium hexahydrate is widely used as a small molecule phosphotyrosine-like substrate in activity assays for protein tyrosine phosphatases. 4-Nitrophenyl phosphate disodium hexahydrate is a colorless substrate that upon hydrolysis is converted to a yellow 4-nitrophenolate ion that can be monitored by absorbance at 405 nm[1].
Related Catalog	Research Areas >> Others Signaling Pathways >> Others >> Others
In Vitro	4-Nitrophenyl phosphate (PNPP) disodium hexahydrate is a commonly used substrate for alkaline phosphatases (ALPs). 4-Nitrophenyl phosphate disodium hexahydrate is hydrolyzed by ALP to PNP (p-nitrophenol), which quenches the fluorescence of novel gold nanoclusters (AuNCs) by the inner filter effect (IFE)[2].
References	[1]. Lountos GT, et al. Structural analysis of human dual-specificity phosphatase 22 complexed with a phosphotyrosine-like substrate. Acta Crystallogr F Struct Biol Commun. 2015;71(Pt 2):199-205. [2]. Qi S, et al. Development of a facile and sensitive method for detecting alkaline phosphatase activity in serum with fluorescent gold nanoclusters based on the inner filter effect. Analyst. 2020;145(11):3871-3877.

Boiling Point	457.8ºC at 760mmHg
Melting Point	>300ºC(lit.)
Molecular Formula	C₆H₁₆NNa₂O₁₂P
Molecular Weight	371.14400
Flash Point	230.7ºC
Exact Mass	371.02100
PSA	183.43000
LogP	2.08010
Appearance	tablets \| white to pale yellow
Storage condition	2-8°C
Water Solubility	H2O: 25 mg/mL at 20 °C, clear, yellow-green

Personal Protective Equipment	Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
Safety Phrases	24/25
RIDADR	NONH for all modes of transport
WGK Germany	3