7BIO

7BIO (7-Bromoindirubin-3-Oxime) is the derivate of indirubin. 7BIO (7-Bromoindirubin-3-Oxime) has inhibitory effects against cyclin-dependent kinase-5 (CDK5) and glycogen synthase kinase-3β (GSK3β). 7BIO (7-Bromoindirubin-3-Oxime) inhibits Aβ oligomer-induced neuroinflammation, synaptic impairments, tau hyper-phosphorylation, activation of astrocytes and microglia, and attenuates Aβ oligomer-induced cognitive impairments in mice[1].

Signaling Pathways >> Stem Cell/Wnt >> GSK-3

Signaling Pathways >> Cell Cycle/DNA Damage >> CDK

Research Areas >> Neurological Disease

Signaling Pathways >> PI3K/Akt/mTOR >> GSK-3

CDK5

GSK3β

7BIO (1 and 10 μM; 24 hours) has neuroprotective effects and can prevent, but cannot rescue Aβ1−42 oligomer-induced cell death in SH-SY5Y cells[1]. Cell Viability Assay[1] Cell Line: SH-SY5Y cells Concentration: 1 and 10 μM Incubation Time: 24 hours Result: Inhibit Aβ oligomer-induced neuronal death. 7BIO prevented, but did not rescue Aβ1−42 oligomer-induced cell death in SH-SY5Y cells.

7Bio (2.3, 7.0, and 23.3 μg/kg; bilateral ventricle injection) significantly attenuates Aβ oligomer-induced impairment of recognition, spatial learning and memory in mice[1]. 7Bio (2.3, 7.0, and 23.3 μg/kg; bilateral ventricle injection) decreases Aβ oligomer-induced increase of TNF-α and IL-6 production in the brain and the expression of synapsin-1 and PSD-95 in the hippocampal region of mice[1]. 7Bio (2.3, 7.0, and 23.3 μg/kg; bilateral ventricle injection) attenuates Aβ oligomer-induced increase expression of pTau, activation of microglia and astrogliosis in the brain of mice[1]. 7Bio (2.3, 7.0, and 23.3 μg/kg; bilateral ventricle injection) prevents decreased expression of pSer9-GSK3β and has no significant effects on the Tau protein level[1]. Animal Model: 8 weeks mice (30 g)[1] Dosage: 2.3, 7.0, and 23.3 μg/kg Administration: bilateral ventricle injection Result: Attenuates Aβ oligomer-induced impairment of recognition, spatial learning and memory in mice.

[1]. Chen L, et al. Indirubin Derivative 7-Bromoindirubin-3-Oxime (7Bio) Attenuates Aβ Oligomer-Induced Cognitive Impairments in Mice. Front Mol Neurosci. 2017;10:393.