MeOSuc-Ala-Ala-Pro-Val-pNA

Names

[ Name ]:
MeOSuc-Ala-Ala-Pro-Val-pNA

[Synonym ]:
MFCD00077137
N-(Methoxysuccinyl)-Ala-Ala-Pro-Val 4-nitroanilide
MeOSuc-Ala-Ala-Pro-Val-p-NA
N-methoxysuccinyl-Ala-Ala-Pro-Val-p-nitroanilide
MeO-Suc-Ala-Ala-Pro-Val-(p-nitroanilide)
N-3-carbomethoxypropanoyl-alanyl-alanyl-prolyl-valine p-nitroanilide
MeOSuc-AAPV-PNA
N-(Methoxysuccinyl)-L-alanyl-L-alanyl-L-prolyl-L-valine 4-nitroanilide
EINECS 232-670-7
MeOSuc-Ala-Ala-Pro-Val-pNA

Biological Activity

[Description]:

MeOSuc-Ala-Ala-Pro-Val-pNA is a sensitive fluorogenic substrate of human leukocyte and porcine pancreatic elastase[1].

[Related Catalog]:

Research Areas >> Others

Signaling Pathways >> Others >> Others

[In Vitro]

MeOSuc-Ala-Ala-Pro-Val-pNA exhibits kc/Km values of 185,000, 15,000, 58,000 and 330,000 M-1s-1 at 0.10 M phosphate, 0.20 M Mes, 0.10 M phosphate and 0.04 M phosphate, respectively, for reactions of human leukocyte elastase[2].

[References]

[1]. Castillo MJ, et al. Sensitive substrates for human leukocyte and porcine pancreatic elastase: a study of the merits of various chromophoric and fluorogenic leaving groups in assays for serine proteases. Anal Biochem. 1979 Oct 15;99(1):53-64.

[2]. Ross L. Stein, et al. Catalysis by human leukocyte elastase. Rate-limiting deacylation for specific p-nitroanilides and amides. J. Am. Chem. Soc. 1984, 106, 3, 796-798.

Chemical & Physical Properties

[ Density]:
1.297g/cm3

[ Boiling Point ]:
908.4ºC at 760 mmHg

[ Molecular Formula ]:
C₂₇H₃₈N₆O₉

[ Molecular Weight ]:
590.62500

[ Flash Point ]:
503.2ºC

[ Exact Mass ]:
590.27000

[ PSA ]:
208.83000

[ LogP ]:
2.33450

[ Index of Refraction ]:
1.567

[ Storage condition ]:
20°C

MSDS

Click to get detail MSDS

Safety Information

[ Personal Protective Equipment ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ Safety Phrases ]:
S22-S24/25

[ RIDADR ]:
NONH for all modes of transport

Synthetic Route

Click to get detail synthetic route

Precursor & DownStream

Precursor

DownStream

Articles

Thrombospondin-1 restrains neutrophil granule serine protease function and regulates the innate immune response during Klebsiella pneumoniae infection.

Mucosal Immunol. 8 , 896-905, (2015)

Neutrophil elastase (NE) and cathepsin G (CG) contribute to intracellular microbial killing but, if left unchecked and released extracellularly, promote tissue damage. Conversely, mechanisms that cons...

Neutrophil extracellular traps directly induce epithelial and endothelial cell death: a predominant role of histones.

PLoS ONE 7 , e32366, (2012)

Neutrophils play an important role in innate immunity by defending the host organism against invading microorganisms. Antimicrobial activity of neutrophils is mediated by release of antimicrobial pept...

Inhibition of elastase by a synthetic cotton-bound serine protease inhibitor: in vitro kinetics and inhibitor release.

Wound Repair Regen. 7(2) , 106-18, (1999)

A cotton-bound serine protease inhibitor of elastase (fiber-inhibitor) has been formulated for in vitro evaluation in chronic wound fluid. As a model to understand the properties of the inhibitor in w...