Fluorescein isothiocyanate

Names

[ Name ]:
Fluorescein isothiocyanate

[Synonym ]:
5-FITC
Isothiocyanatofluorescein
5-Isothionatofluorescein
Benzoic acid, 2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)-5-isothiocyanato-
FITC Fluorescein 5-isothiocyanate
EINECS 248-207-7
Fluorescein Isothiosyanate
fluorescein isothiocyanate isomer 1
fluorescein 6-isothiocyanate
FITC ISOMER
Fluorescein 5-Isothiocyanate (isomer I)
3',6'-Dihydroxy-5-isothiocyanato-3H-spiro[2-benzofurane-1,9'-xanthén]-3-one
5-FITC (isomer I)
5-Isothiocyanatofluorescein (isomer I)
FITC
5-Fluorescein Isothiocyanate
fluorescein 5-isothiocyanate
FITC,Fluorescein 5-isothiocyanate
FITC isomer I
Fluorescein isothiocyanate,isomer 1
2-(6-Hydroxy-3-oxo-3H-xanthen-9-yl)-5-isothiocyanatobenzoic acid
Fluorescein Isothiocyanate (isomer I)
Fluorescein isothiocyanate isomer I
Spiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one, 3',6'-dihydroxy-5-isothiocyanato-
FITC-I
Fluorescein 5(6)-isothiocyanate
Fluorescein-5-isothiocyanate
MFCD00005063
Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 3',6'-dihydroxy-5-isothiocyanato-
Fluorescein Isothiocyanate
3',6'-Dihydroxy-5-isothiocyanato-3H-spiro[2-benzofuran-1,9'-xanthen]-3-one

Biological Activity

[Description]:

FITC is a derivative of fluorescein for the labeling of amines.

[Related Catalog]:

Signaling Pathways >> Others >> Others

Dye Reagents

Research Areas >> Others

[In Vitro]

Annexin V-FITC/PI is employed to detect chondrocyte apoptosis[1]. The PEI-modified CDs are conjugated with fluorescein isothiocyanate (FITC) to fabricate CDs-FITC composites[2].

[Cell Assay]

The frequency of chondrocyte apoptosis is measured by flow cytometry with Annexin V-fluorescein isothiocyanate (FITC) and propidium iodide. A total of 1×104 treated chondrocytes are collected from each group, washed in cold PBS and incubated with Annexin V-FITC and PI at room temperature for 15 min in the dark on ice. These samples are then analyzed using a fluorescence-activated cell sorter. Cell Quest software is used to analyze the percentage of apoptosis. All tests are repeated in triplicate.

[References]

[1]. Gao SG, et al. Phosphorylation of osteopontin has proapoptotic and proinflammatory effects on human knee osteoarthritis chondrocytes. Exp Ther Med. 2016 Nov;12(5):3488-3494. Epub 2016 Oct 5.

[2]. Zhu X, et al. Ratiometric, visual, dual-signal fluorescent sensing and imaging of pH/copper ions in real samples based on carbon dots-fluorescein isothiocyanate composites. Talanta. 2017 Jan 1;162:65-71

[Related Small Molecules]

Chemical & Physical Properties

[ Density]:
1.5±0.1 g/cm3

[ Boiling Point ]:
708.6±60.0 °C at 760 mmHg

[ Melting Point ]:
>360 °C(lit.)

[ Molecular Formula ]:
C₂₁H₁₁NO₅S

[ Molecular Weight ]:
389.381

[ Flash Point ]:
382.4±32.9 °C

[ Exact Mass ]:
389.035797

[ PSA ]:
120.44000

[ LogP ]:
4.00

[ Vapour Pressure ]:
0.0±2.3 mmHg at 25°C

[ Index of Refraction ]:
1.754

[ Storage condition ]:
2-8°C

[ Stability ]:
Stability Stable, but moisture-sensitive. Incompatible with strong oxidizing agents, strong bases, amines, acids.

[ Water Solubility ]:
practically insoluble

MSDS

Click to get detail MSDS

Safety Information

[ Symbol ]:

GHS08

[ Signal Word ]:
Danger

[ Hazard Statements ]:
H317-H334

[ Precautionary Statements ]:
P261-P280-P342 + P311

[ Personal Protective Equipment ]:
dust mask type N95 (US);Eyeshields;Faceshields;Gloves

[ Hazard Codes ]:
Xn: Harmful;

[ Risk Phrases ]:
R22;R36/37/38;R42/43

[ Safety Phrases ]:
S22-S45-S36/37/39-S26-S36/37

[ RIDADR ]:
NONH for all modes of transport

[ WGK Germany ]:
3

Synthetic Route

Click to get detail synthetic route

Precursor & DownStream

Precursor

DownStream

Articles

Imaging of a clinically relevant stroke model: glucose hypermetabolism revisited.

Stroke 46(3) , 835-42, (2015)

Ischemic stroke has been shown to cause hypermetabolism of glucose in the ischemic penumbra. Experimental and clinical data indicate that infarct-related systemic hyperglycemia is a potential therapeu...

Surface modifications of silica nanoparticles are crucial for their inert versus proinflammatory and immunomodulatory properties.

Int. J. Nanomedicine 9 , 2815-32, (2014)

Silica (SiO₂) nanoparticles (NPs) are widely used in diverse industrial and biomedical applications. Their applicability depends on surface modifications, which can limit potential health problems.To ...

Uptake of neutrophil-derived Ym1 protein distinguishes wound macrophages in the absence of interleukin-4 signaling in murine wound healing.

Am. J. Pathol. 184(12) , 3249-61, (2014)

The determination of regenerative wound-healing macrophages as alternatively activated macrophages is currently questioned by the absence of IL-4 in wound tissue. Yet, murine wound tissue expressed hi...