Fluorescein

Names

[ Name ]:
Fluorescein

[Synonym ]:
D and C Yellow No. 7
D&C Yellow No. 7
D+C Yellow No. 7
Fluorescein (free acid)
3',6'-Dihydroxyspiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one
3',6'-Fluorandiol
Fluorecein
Eosin
YELLOW 7
3',6'-Dihydroxy-3H-spiro[isobenzofuran-1,9'-xanthen]-3-one
KI201
MFCD00005050
Acid Yellow 73
3',6'-Dihydroxy-3H-spiro[2-benzofuran-1,9'-xanthen]-3-one
Fluorescite
EINECS 219-031-8
Uranine Yellow
C.I. Acid Yellow 73
angiofluor
Soluble fluorescein
D & C Yellow no. 7
Fluor-I-Strip
D & C Yellow no. 8
Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 3',6'-dihydroxy-
Fluorescein
Ful-Glo
Funduscein

Biological Activity

[Description]:

Fluorescein is a synthetic fluorescent tracer.

[Related Catalog]:

Signaling Pathways >> Others >> Others

Dye Reagents

Research Areas >> Others

[In Vitro]

The fluorescein dye is a common fluorescent probe. Its very high molar absorptivity at the wavelength of the argon laser (488 nm), large fluorescence quantum yield and high photostability makes it a very useful and sensitive fluorescent label. Fluorescein is commercially available in many derivatives, such as fluorescein isothiocyanate and fluorescein succinimidyl ester, that can be covalently attached to macromolecules and to amino acids. The labeled molecules can be detected with very high sensitivity which is utilized in, for example, capillary electrophoresis. The emission spectrum of fluorescein overlaps extensively the absorption spectrum of tetramethyl rhodamine, which is a related strongly fluorescent dye, making this pair very suitable for energy transfer experiments to determine distances within and between labeled macromolecules. Fluorescein in aqueous solution occurs in cationic, neutral, anionic and dianionic forms making its absorption and fluoresence properties strongly pH dependent[1].

[References]

[1]. R Sjöback, et al. Absorption and fluorescence properties of fluorescein. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy

[Related Small Molecules]

Chemical & Physical Properties

[ Density]:
1.6±0.1 g/cm3

[ Boiling Point ]:
620.8±55.0 °C at 760 mmHg

[ Melting Point ]:
320 °C(lit.)

[ Molecular Formula ]:
C₂₀H₁₂O₅

[ Molecular Weight ]:
332.306

[ Flash Point ]:
232.6±25.0 °C

[ Exact Mass ]:
332.068481

[ PSA ]:
87.74000

[ LogP ]:
2.98

[ Vapour Pressure ]:
0.0±1.9 mmHg at 25°C

[ Index of Refraction ]:
1.792

[ Storage condition ]:
−20°C

[ Water Solubility ]:
insoluble

MSDS

Click to get detail MSDS

Toxicological Information

CHEMICAL IDENTIFICATION

RTECS NUMBER :: LM5075000

CHEMICAL NAME :: Fluorescein

CAS REGISTRY NUMBER :: 2321-07-5

BEILSTEIN REFERENCE NO. :: 0094324

LAST UPDATED :: 199707

DATA ITEMS CITED :: 10

MOLECULAR FORMULA :: C20-H12-O5

MOLECULAR WEIGHT :: 332.32

WISWESSER LINE NOTATION :: T C666 BO EVJ IR BVQ& MQ

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

TYPE OF TEST :: LDLo - Lowest published lethal dose

ROUTE OF EXPOSURE :: Intraperitoneal

SPECIES OBSERVED :: Rodent - rat

DOSE/DURATION :: 600 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intravenous

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 300 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :: LDLo - Lowest published lethal dose

ROUTE OF EXPOSURE :: Unreported

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 600 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :: LDLo - Lowest published lethal dose

ROUTE OF EXPOSURE :: Intravenous

SPECIES OBSERVED :: Rodent - rabbit

DOSE/DURATION :: 300 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :: LDLo - Lowest published lethal dose

ROUTE OF EXPOSURE :: Unreported

SPECIES OBSERVED :: Rodent - guinea pig

DOSE/DURATION :: 400 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

MUTATION DATA

TYPE OF TEST :: DNA adduct

TEST SYSTEM :: Bacteria - Escherichia coli

DOSE/DURATION :: 15 umol/L

REFERENCE :: MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 89,95,1981 *** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA *** NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA : NOHS - National Occupational Hazard Survey (1974) NOHS Hazard Code - 33350 No. of Facilities: 495 (estimated) No. of Industries: 18 No. of Occupations: 21 No. of Employees: 5188 (estimated) NOES - National Occupational Exposure Survey (1983) NOES Hazard Code - 33350 No. of Facilities: 2171 (estimated) No. of Industries: 42 No. of Occupations: 55 No. of Employees: 88495 (estimated) No. of Female Employees: 21558 (estimated)

Safety Information

[ Symbol ]:

GHS07

[ Signal Word ]:
Warning

[ Hazard Statements ]:
H319

[ Precautionary Statements ]:
P305 + P351 + P338

[ Personal Protective Equipment ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ Hazard Codes ]:
Xi:Irritant;

[ Risk Phrases ]:
R36;R43

[ Safety Phrases ]:
S22-S24/25-S37/39-S26

[ RIDADR ]:
NONH for all modes of transport

[ WGK Germany ]:
3

[ RTECS ]:
LM5075000

[ Packaging Group ]:
I; II; III

[ HS Code ]:
3204200000

Synthetic Route

Click to get detail synthetic route

Precursor & DownStream

Precursor

DownStream

Customs

[ HS Code ]: 2932999099

[ Summary ]:
2932999099. other heterocyclic compounds with oxygen hetero-atom(s) only. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0%

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