Thiazolyl Blue

Names

[ CAS No. ]:
298-93-1

[ Name ]:
Thiazolyl Blue

[Synonym ]:
3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium Bromide
ThiazolylBlue(Mtt)
MttA.R.
2H-Tetrazolium, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-, bromide (1:1)
3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium Bromide
3-(4,5-Dimethyl-1,3-thiazol-2-yl)-2,5-diphenyl-2H-tetrazol-3-ium bromide
Methylthiazoletetrazolium bromide
Thiazolyl Blue
2H-tetrazolium, 2-(4,5-dimethyl-2-thiazolyl)-3,5-diphenyl-, bromide (1:1)
2-(4,5-Dimethyl-1,3-thiazol-2-yl)-3,5-diphenyl-2H-tetrazol-3-ium bromide
MFCD00011964
EINECS 206-069-5
THIAZOLE BLUE
Thiazolyl blue tetrazolium bromide (MTT)
thiazolyl blue tetrazolium bromide
mmttetrazolium
3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyl-2H-Tetrazolium Bromide
MTT

Biological Activity

[Description]:

MTT is a colorimetric agent widely used to measure cell proliferation. MTT is reduced from yellow color to purple formazan in living cells.

[Related Catalog]:

Signaling Pathways >> Others >> Others

Biochemical Assay Reagents

Research Areas >> Others

[In Vitro]

MTT is used in conjunction with rhodamine B to measure mitochondrial membrane potential. MTT-formazan is generated in mitochondria acts as a fluorescence quencher for rhodamine that distributes across membranes of viable cells according to membrane potential. In the absence of mPMS, cellular reduction of MTT is strong. MTT is membrane-impermeable when incorporated into large unilamellar liposomes, and therefore it is taken up by cells via endocytosis[1].

[References]

[1]. Berridge MV, et al. Tetrazolium dyes as tools in cell biology: new insights into their cellular reduction. Biotechnol Annu Rev. 2005;11:127-52.

[Related Small Molecules]

Chemical & Physical Properties

[ Melting Point ]:
195 °C (dec.)(lit.)

[ Molecular Formula ]:
C₁₈H₁₆BrN₅S

[ Molecular Weight ]:
414.322

[ Exact Mass ]:
413.030975

[ PSA ]:
75.72000

[ LogP ]:
0.28830

[ Storage condition ]:
2-8°C

[ Water Solubility ]:
Slightly soluble

MSDS

Click to get detail MSDS

Toxicological Information

CHEMICAL IDENTIFICATION

RTECS NUMBER :: XF8060000

CHEMICAL NAME :: 2H-Tetrazolium, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-, bromide

CAS REGISTRY NUMBER :: 298-93-1

LAST UPDATED :: 199701

DATA ITEMS CITED :: 4

MOLECULAR FORMULA :: C18-H16-N5-S.Br

MOLECULAR WEIGHT :: 414.36

WISWESSER LINE NOTATION :: T5NNKNJ BR& ER& C- BT5N CSJ D1 E1 &E

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

MUTATION DATA

TYPE OF TEST :: Mutation in microorganisms

TEST SYSTEM :: Bacteria - Escherichia coli

DOSE/DURATION :: 5 ug/plate

REFERENCE :: MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 68,107,1979 *** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA *** NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA : NOES - National Occupational Exposure Survey (1983) NOES Hazard Code - X1328 No. of Facilities: 93 (estimated) No. of Industries: 1 No. of Occupations: 3 No. of Employees: 1106 (estimated) No. of Female Employees: 985 (estimated)

Safety Information

[ Symbol ]:

GHS07, GHS08

[ Signal Word ]:
Warning

[ Hazard Statements ]:
H315-H319-H335-H341

[ Precautionary Statements ]:
P261-P281-P305 + P351 + P338

[ Personal Protective Equipment ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ Hazard Codes ]:
Xi: Irritant;

[ Risk Phrases ]:
R46

[ Safety Phrases ]:
S22-S24/25

[ RIDADR ]:
NONH for all modes of transport

[ WGK Germany ]:
3

[ RTECS ]:
XF8060000

[ HS Code ]:
29341000

Customs

[ HS Code ]: 29341000

Articles

Neuroprotective effect of modified Chungsimyeolda-tang, a traditional Korean herbal formula, via autophagy induction in models of Parkinson's disease.

J. Ethnopharmacol. 159 , 93-101, (2014)

Previous studies in our laboratory revealed the neuroprotective effect of modified Yeoldahanso-tang (MYH) in models of Parkinson׳s disease (PD). In this study, we investigated another traditional Kore...

Pro-apoptotic and pro-autophagic effects of the Aurora kinase A inhibitor alisertib (MLN8237) on human osteosarcoma U-2 OS and MG-63 cells through the activation of mitochondria-mediated pathway and inhibition of p38 MAPK/PI3K/Akt/mTOR signaling pathway.

Drug Des. Devel. Ther. 9 , 1555-84, (2015)

Osteosarcoma (OS) is the most common malignant bone tumor occurring mostly in children and adolescents between 10 and 20 years of age with poor response to current therapeutics. Alisertib (ALS, MLN823...

Alisertib, an Aurora kinase A inhibitor, induces apoptosis and autophagy but inhibits epithelial to mesenchymal transition in human epithelial ovarian cancer cells.

Drug Des. Devel. Ther. 9 , 425-64, (2015)

Ovarian cancer is a leading killer of women, and no cure for advanced ovarian cancer is available. Alisertib (ALS), a selective Aurora kinase A (AURKA) inhibitor, has shown potent anticancer effects, ...