Boc-Lys(Ac)-AMC

Names

[ Name ]:
Boc-Lys(Ac)-AMC

[Synonym ]:
(S)-tert-Butyl (6-acetamido-1-((4-methyl-2-oxo-2H-chromen-7-yl)amino)-1-oxohexan-2-yl)carbamate
Histone Deacetylase Substrate,Fluorogenic
Nalpha-Boc-Nepsilon-acetyl-L-lysine 7-amido-4-methylcoumarin
N-(4-Methyl-7-coumarinyl)-Nalpha-(t-butoxycarbonyl)-Nomega-acetyllysinamide
Boc-Ac-Lys-AMC
Boc-Lys(Ac)-AMC
Boc-L(Ac)-AMC
Boc-Lys(Ac)-7-Amino-4-Methylcoumarin
tert-Boc (N-acetyl-Lys)-AMC

Biological Activity

[Description]:

Boc-Lys(Ac)-AMC is a cell-permeable fluorometric HDAC substrate (Ex/Em = 355 nm/460 nm)[1][2].

[Related Catalog]:

Research Areas >> Others

Signaling Pathways >> Cell Cycle/DNA Damage >> HDAC

Signaling Pathways >> Epigenetics >> HDAC

[In Vitro]

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). 基于组织培养的 HDAC 检测[2] 1. 将 6 × 104 细胞在 100 mL 培养基中加入 25 μM Boc-Lys(Ac)-AMC 于 96 孔组织培养板中培养过夜。 2. 37°C，5% CO2 下孵育 2-3 小时。 3. 加入含有细胞裂解缓冲液和胰蛋白酶试剂的 HADC 显影液，终止脱乙酰反应。 4. 室温孵育 15 分钟。 5. 在 Ex/Em = 355 nm/460 nm 处，用 SpectraMax M5 微板仪测量 AMC 的荧光信号。

[References]

[1]. Reddy DS, et al. Measuring Histone Deacetylase Inhibition in the Brain. Curr Protoc Pharmacol. 2018 Jun;81(1):e41.

[2]. Ueki N, et al. Selective cancer targeting with prodrugs activated by histone deacetylases and a tumour-associated protease. Nat Commun. 2013;4:2735.

Chemical & Physical Properties

[ Molecular Formula ]:
C₂₃H₃₁N₃O₆

[ Molecular Weight ]:
445.50900

[ Exact Mass ]:
445.22100

[ PSA ]:
126.74000

[ LogP ]:
4.09440

Safety Information

[ Hazard Codes ]:
Xi

Synthetic Route

Click to get detail synthetic route

Precursor & DownStream

Precursor

DownStream

Related Compounds

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