ALDH1A3-IN-1

ALDH1A3-IN-1 (Compound 14) is a potent ALDH1A3 inhibitor, with an IC50 of 0.63 µM and a Ki of 0.46 µM. ALDH1A3-IN-1 can be studied in prostate cancer[1].

Signaling Pathways >> Metabolic Enzyme/Protease >> Aldehyde Dehydrogenase (ALDH)

Research Areas >> Cancer

ALDH1A3:0.63 μM (IC50)

ALDH1A1:7.08 μM (IC50)

ALDH3A1:8.00 μM (IC50)

ALDH1A3:0.46 μM (Ki)

ALDH1A3:26.92 μM (Km)

ALDH1A3-IN-1 (Compound 14) (0-200 µM, 96 h) shows antiproliferative activity in prostate cancer cell lines[1]. ALDH1A3-IN-1 (0-200 µM, 72 h) reduces cell viability of primary prostate cells in a dose-dependent manner[1]. Cell Proliferation Assay[1] Cell Line: PC3, LNCaP, and DU145 cells Concentration: 12.5-200 µM Incubation Time: 96 h Result: Showed antiproliferative activity with IC50 values of 47 ± 6, 25 ± 1, and 61 ± 5 µM against PC3, LNCaP, and DU145 cells. Cell Viability Assay[1] Cell Line: Primary cells cultured at early passage from human prostate tissue biopsies Concentration: 50 and 200 μM Incubation Time: 72 h Result: Resulted a dose-dependent reduction in percentage cell viability of primary prostate epithelial cultures.

[1]. Ali I M Ibrahim, et al. Expansion of the 4-(Diethylamino)benzaldehyde Scaffold to Explore the Impact on Aldehyde Dehydrogenase Activity and Antiproliferative Activity in Prostate Cancer. J Med Chem. 2022 Mar 10;65(5):3833-3848.