5-CFDA,AM

Names

[ Name ]:
5-CFDA,AM

[Synonym ]:
5-CFDA,AM
CFDA-AM
acetoxymethyl ester
5-Carboxyfluorescein diacetate acetoxymethyl ester
5-carboxyfluorescein diacetate

Biological Activity

[Description]:

5-CFDA-AM is a cell-permeable esterase substrate that can be used as an active probe to measure enzyme activity and cell membrane integrity. 5-CFDA-AM is electroneutral and can enter the cell at a lower concentration than CFDA, where it is hydrolysed by intracellular esterases to produce carboxyfluorescein. Carboxyfluorescein contains an additional negative charge and can be better retained in the cell[1].

[Related Catalog]:

Research Areas >> Others

Signaling Pathways >> Others >> Others

[References]

[1]. Elena Gorokhova, et al. A comparison of TO-PRO-1 iodide and 5-CFDA-AM staining methods for assessing viability of planktonic algae with epifluorescence microscopy. J Microbiol Methods. 2012 Jun;89(3):216-21.

Chemical & Physical Properties

[ Density]:
1.51g/cm3

[ Boiling Point ]:
737ºC at 760mmHg

[ Molecular Formula ]:
C₂₈H₂₀O₁₁

[ Molecular Weight ]:
532.45200

[ Flash Point ]:
312.7ºC

[ Exact Mass ]:
532.10100

[ PSA ]:
140.73000

[ LogP ]:
3.78260

[ Vapour Pressure ]:
1.39E-21mmHg at 25°C

[ Index of Refraction ]:
1.655

Related Compounds

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