124412-00-6

124412-00-6 structure

Name: 5-CFDA,AM
Chemical Name: acetyloxymethyl 3',6'-diacetyloxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-carboxylate
CAS Number: 124412-00-6
Molecular Formula: C₂₈H₂₀O₁₁
Molecular Weight: 532.45200
Catalog: Research Areas Others
Create Date: 2018-06-17 14:19:32
Modify Date: 2025-08-25 15:18:45
5-CFDA-AM is a cell-permeable esterase substrate that can be used as an active probe to measure enzyme activity and cell membrane integrity. 5-CFDA-AM is electroneutral and can enter the cell at a lower concentration than CFDA, where it is hydrolysed by intracellular esterases to produce carboxyfluorescein. Carboxyfluorescein contains an additional negative charge and can be better retained in the cell[1].

Name	acetyloxymethyl 3',6'-diacetyloxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-carboxylate
Synonyms	5-CFDA,AM CFDA-AM acetoxymethyl ester 5-Carboxyfluorescein diacetate acetoxymethyl ester 5-carboxyfluorescein diacetate

Description	5-CFDA-AM is a cell-permeable esterase substrate that can be used as an active probe to measure enzyme activity and cell membrane integrity. 5-CFDA-AM is electroneutral and can enter the cell at a lower concentration than CFDA, where it is hydrolysed by intracellular esterases to produce carboxyfluorescein. Carboxyfluorescein contains an additional negative charge and can be better retained in the cell[1].
Related Catalog	Research Areas >> Others Signaling Pathways >> Others >> Others
References	[1]. Elena Gorokhova, et al. A comparison of TO-PRO-1 iodide and 5-CFDA-AM staining methods for assessing viability of planktonic algae with epifluorescence microscopy. J Microbiol Methods. 2012 Jun;89(3):216-21.