Indo-1 AM

Names

[ CAS No. ]:
112926-02-0

[ Name ]:
Indo-1 AM

[Synonym ]:
MFCD00036955
Indo-1 pentaacetoxymethyl ester
INDO 1/AM
Indo-1 AM

Biological Activity

[Description]:

Indo-1 AM is a fluorescent Ca2+ indicator (λex=340 nm, λem=405/485 nm).

[Related Catalog]:

Signaling Pathways >> Others >> Others

Dye Reagents

Research Areas >> Others

[In Vitro]

Indo-1 AM is a fluorescent Ca2+ indicator with λex=340 nm and λem=405/485 nm[1]. In rabbit hearts that are exposed to Indo-1 AM, 72% of the dye is in the cytosol, and the remainder is associated with the mitochondria, microsomes and the nucleus[2].

[Cell Assay]

The cells are loaded with indo-I and fura-2 by exposure to the membrane-permeant forms, Indo-1 AM (indo-l-acetoxymethyl ester) and fura-2-acetoxymethyl ester, respectively. A mixture of 10 μL of 1 mM Indo-1 AM or fura-2/AM in dimethylsulfoxide (DMSO), 2.5 μL of 25% wt/wt Pluronic F-127 in DMSO, and 75 μL fetal calf serum (FCS) or newborn calf serum (NCS) is added to 2 mL Tyrode solution containing the cells and 40 μL FCS or NCS. Loading is achieved by gently shaking the cells for 15 to 30 min[2].

[References]

[1]. Andrienko T, et al. Real-Time Fluorescence Measurements of ROS and [Ca2+] in Ischemic / Reperfused Rat Hearts: Detectable Increases Occur only after Mitochondrial Pore Opening and Are Attenuated by Ischemic Preconditioning. PLoS One. 2016 Dec 1;11(12):e0167300.

[2]. Blatter LA, et al. Intracellular diffusion, binding, and compartmentalization of the fluorescent calcium indicators indo-1 and fura-2. Biophys J. 1990 Dec;58(6):1491-9.

[Related Small Molecules]

Chemical & Physical Properties

[ Density]:
1.374g/cm3

[ Boiling Point ]:
1009.2ºC at 760mmHg

[ Molecular Formula ]:
C₄₇H₅₁N₃O₂₂

[ Molecular Weight ]:
1009.91000

[ Flash Point ]:
564.1ºC

[ Exact Mass ]:
1009.30000

[ PSA ]:
303.73000

[ LogP ]:
3.14650

[ Vapour Pressure ]:
3.2E-28mmHg at 25°C

[ Index of Refraction ]:
1.577

Safety Information

[ Personal Protective Equipment ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ Safety Phrases ]:
S22-S24/25

[ RIDADR ]:
NONH for all modes of transport

Articles

Quantitative TCR:pMHC Dissociation Rate Assessment by NTAmers Reveals Antimelanoma T Cell Repertoires Enriched for High Functional Competence.

J. Immunol. 195 , 356-66, (2015)

Experimental models demonstrated that therapeutic induction of CD8 T cell responses may offer protection against tumors or infectious diseases providing that T cells have sufficiently high TCR/CD8:pMH...

Identification of Rare High-Avidity, Tumor-Reactive CD8+ T Cells by Monomeric TCR-Ligand Off-Rates Measurements on Living Cells.

Cancer Res. 75 , 1983-91, (2015)

The avidity of the T-cell receptor (TCR) for antigenic peptides presented by the peptide-MHC (pMHC) on cells is a key parameter for cell-mediated immunity. Yet a fundamental feature of most tumor anti...

Pharmacological analysis by HOE642 and KB-R9032 of the role of Na(+)/H(+) exchange in the endothelin-1-induced Ca(2+) signalling in rabbit ventricular myocytes.

Br. J. Pharmacol. 131(3) , 638-44, (2000)

The role of Na(+)/H(+) exchange in endothelin-1 (ET-1)-induced increases in Ca(2+) transients and cell shortening was studied in rabbit ventricular myocytes loaded with indo-1/AM. Selective inhibitors...

Related Compounds

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