Shanghai Nianxing Industrial Co., Ltd
China
Product Name: Indo-1 AM (Indo-1 Acetoxymethyl ester)
Price: Check
Purity: 98.0%
Stocking Period: 10 Day
Contact: Yang
Email: sales@echemcloud.com

112926-02-0

112926-02-0 structure

112926-02-0 structure

Name: Indo-1 AM
Chemical Name: acetyloxymethyl 2-[4-[bis[2-(acetyloxymethoxy)-2-oxoethyl]amino]-3-[2-[2-[bis[2-(acetyloxymethoxy)-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]phenyl]-1H-indole-6-carboxylate
CAS Number: 112926-02-0
Molecular Formula: C₄₇H₅₁N₃O₂₂
Molecular Weight: 1009.91000
Catalog: Dye Reagents
Create Date: 2018-03-03 08:00:00
Modify Date: 2024-01-24 02:08:11
Indo-1 AM is a fluorescent Ca2+ indicator (λex=340 nm, λem=405/485 nm).

Name	acetyloxymethyl 2-[4-[bis[2-(acetyloxymethoxy)-2-oxoethyl]amino]-3-[2-[2-[bis[2-(acetyloxymethoxy)-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]phenyl]-1H-indole-6-carboxylate
Synonyms	MFCD00036955 Indo-1 pentaacetoxymethyl ester INDO 1/AM Indo-1 AM

Description	Indo-1 AM is a fluorescent Ca2+ indicator (λex=340 nm, λem=405/485 nm).
Related Catalog	Signaling Pathways >> Others >> Others Dye Reagents Research Areas >> Others
In Vitro	Indo-1 AM is a fluorescent Ca2+ indicator with λex=340 nm and λem=405/485 nm[1]. In rabbit hearts that are exposed to Indo-1 AM, 72% of the dye is in the cytosol, and the remainder is associated with the mitochondria, microsomes and the nucleus[2].
Cell Assay	The cells are loaded with indo-I and fura-2 by exposure to the membrane-permeant forms, Indo-1 AM (indo-l-acetoxymethyl ester) and fura-2-acetoxymethyl ester, respectively. A mixture of 10 μL of 1 mM Indo-1 AM or fura-2/AM in dimethylsulfoxide (DMSO), 2.5 μL of 25% wt/wt Pluronic F-127 in DMSO, and 75 μL fetal calf serum (FCS) or newborn calf serum (NCS) is added to 2 mL Tyrode solution containing the cells and 40 μL FCS or NCS. Loading is achieved by gently shaking the cells for 15 to 30 min[2].
References	[1]. Andrienko T, et al. Real-Time Fluorescence Measurements of ROS and [Ca2+] in Ischemic / Reperfused Rat Hearts: Detectable Increases Occur only after Mitochondrial Pore Opening and Are Attenuated by Ischemic Preconditioning. PLoS One. 2016 Dec 1;11(12):e0167300. [2]. Blatter LA, et al. Intracellular diffusion, binding, and compartmentalization of the fluorescent calcium indicators indo-1 and fura-2. Biophys J. 1990 Dec;58(6):1491-9.

Density	1.374g/cm3
Boiling Point	1009.2ºC at 760mmHg
Molecular Formula	C₄₇H₅₁N₃O₂₂
Molecular Weight	1009.91000
Flash Point	564.1ºC
Exact Mass	1009.30000
PSA	303.73000
LogP	3.14650
Vapour Pressure	3.2E-28mmHg at 25°C
Index of Refraction	1.577

Personal Protective Equipment	Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
Safety Phrases	S22-S24/25
RIDADR	NONH for all modes of transport

The content on this webpage is sourced from various professional data sources. If you have any questions or concerns regarding the content, please feel free to contact service1@chemsrc.com.