N-Methylisatoic Anhydride

Names

[ Name ]:
N-Methylisatoic Anhydride

[Synonym ]:
1-Methyl-1H-benzo[d][1,3]oxazine-2,4-dione
N-Methylisatoic Anhydride
MFCD00006815
1-methyl-3,1-benzoxazine-2,4-dione
EINECS 233-714-8

Biological Activity

[Description]:

N-Methylisatoic anhydride (NMIA) is a 2'-OH selective acylation agent of RNAs, and is widely used forresolving secondary RNA structures using the SHAPE (Selective 2'-Hydroxyl Acylation Analyzed by Primer Extension) technology[1].

[Related Catalog]:

Research Areas >> Others

Signaling Pathways >> Others >> Others

[References]

[1]. Ursuegui S, et al. Biotin-conjugated N-methylisatoic anhydride: a chemical tool for nucleic acid separation by selective 2'-hydroxyl acylation of RNA. Chem Commun (Camb). 2014 Jun 1;50(43):5748-51.

Chemical & Physical Properties

[ Density]:
1.348 g/cm3

[ Boiling Point ]:
297.5ºC at 760 mmHg

[ Melting Point ]:
165 °C (dec.)(lit.)

[ Molecular Formula ]:
C₉H₇NO₃

[ Molecular Weight ]:
177.15700

[ Flash Point ]:
133.7ºC

[ Exact Mass ]:
177.04300

[ PSA ]:
52.21000

[ LogP ]:
0.49170

[ Vapour density ]:
6.1 (vs air)

[ Vapour Pressure ]:
0.00135mmHg at 25°C

[ Index of Refraction ]:
1.583

[ Storage condition ]:
-20°C

MSDS

Click to get detail MSDS

Toxicological Information

CHEMICAL IDENTIFICATION

RTECS NUMBER :: DM3140000

CHEMICAL NAME :: 2H-3,1-Benzoxazine-2,4(1H)-dione, 1-methyl-

CAS REGISTRY NUMBER :: 10328-92-4

BEILSTEIN REFERENCE NO. :: 0383957

LAST UPDATED :: 199701

DATA ITEMS CITED :: 2

MOLECULAR FORMULA :: C9-H7-N-O3

MOLECULAR WEIGHT :: 177.17

WISWESSER LINE NOTATION :: T66 BNVOVJ B1

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

TYPE OF TEST :: TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :: Oral

DOSE :: 34800 mg/kg

SEX/DURATION :: male 8 day(s) pre-mating female 21 day(s) pre-mating

TOXIC EFFECTS :: Reproductive - Fertility - female fertility index (e.g. # females pregnant per # sperm positive females; # females pregnant per # females mated)

REFERENCE :: MPHEAE Medicina et Pharmacologia Experimentalis. (Basel, Switzerland) V.12-17, 1965-67. For publisher information, see PHMGBN. Volume(issue)/page/year: 15,7,1966

Safety Information

[ Symbol ]:

GHS07

[ Signal Word ]:
Warning

[ Hazard Statements ]:
H315-H319-H335

[ Precautionary Statements ]:
P261-P305 + P351 + P338

[ Personal Protective Equipment ]:
dust mask type N95 (US);Eyeshields;Gloves

[ Hazard Codes ]:
Xi:Irritant;

[ Risk Phrases ]:
R36/37/38

[ Safety Phrases ]:
S26-S37/39

[ RIDADR ]:
NONH for all modes of transport

[ WGK Germany ]:
3

[ RTECS ]:
DM3140000

[ HS Code ]:
2934999090

Synthetic Route

Click to get detail synthetic route

Precursor & DownStream

Precursor

DownStream

Customs

[ HS Code ]: 2934999090

[ Summary ]:
2934999090. other heterocyclic compounds. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0%

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