Peptide bond formation by aminolysin-A catalysis: a simple approach to enzymatic synthesis of diverse short oligopeptides and biologically active puromycins

…, M Iwabuchi, S Miyoshi, T Nitoda, T Hatanaka

Index: Usuki, Hirokazu; Yamamoto, Yukihiro; Arima, Jiro; Iwabuchi, Masaki; Miyoshi, Shozo; Nitoda, Teruhiko; Hatanaka, Tadashi Organic and Biomolecular Chemistry, 2011 , vol. 9, # 7 p. 2327 - 2335

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Citation Number: 10

Abstract

A new S9 family aminopeptidase derived from the actinobacterial thermophile Acidothermus cellulolyticus was cloned and engineered into a transaminopeptidase by site-directed mutagenesis of catalytic Ser491 into Cys. The engineered biocatalyst, designated aminolysin-A, can catalyze the formation of peptide bonds to give linear homo- oligopeptides, hetero-dipeptides, and cyclic dipeptides using cost-effective substrates in a ...