Microbiology 2011-09-01

Microbial competition between Bacillus subtilis and Staphylococcus aureus monitored by imaging mass spectrometry.

David J Gonzalez, Nina M Haste, Andrew Hollands, Tinya C Fleming, Matthew Hamby, Kit Pogliano, Victor Nizet, Pieter C Dorrestein

Index: Microbiology 157(Pt 9) , 2485-92, (2011)

Full Text: HTML

Abstract

Microbial competition exists in the general environment, such as soil or aquatic habitats, upon or within unicellular or multicellular eukaryotic life forms. The molecular actions that govern microbial competition, leading to niche establishment and microbial monopolization, remain undetermined. The emerging technology of imaging mass spectrometry (IMS) enabled the observation that there is directionality in the metabolic output of the organism Bacillus subtilis when co-cultured with Staphylococcus aureus. The directionally released antibiotic alters S. aureus virulence factor production and colonization. Therefore, IMS provides insight into the largely hidden nature of competitive microbial encounters and niche establishment, and provides a paradigm for future antibiotic discovery.