Biochemical and Biophysical Research Communications 1983-03-16

Photoaffinity reagents for use with pepsin and other carboxyl proteases.

S H Hixson, J L Hurwitz, K J Langridge, D C Nichols, K M Provost, A M Wolff

Index: Biochem. Biophys. Res. Commun. 111(2) , 630-5, (1983)

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Abstract

Two compounds have been designed to serve as photoaffinity reagents for use with carboxyl proteases. 1,2-Epoxy-3-(4'-azido-2'-nitrophenoxy)propane has been synthesized and shown to react with porcine pepsin in the same fashion as the traditional inhibitor 1,2-epoxy-3-(p-nitrophenoxy)propane, while p-azidophenacyl bromide is similar to other phenacyl bromides in its reaction with pepsin. In combination with p-azido-alpha-diazoacetophenone, previously shown to resemble alpha-diazo carbonyl reagents in its reaction with pepsin, photoaffinity analogs are now available for all three of the widely-used carboxyl protease inhibitors.