Biochemical and Biophysical Research Communications 1980-11-17

Stationary ultrafiltration: a radio-tracer technique for studying stereoselective binding of racemic ligands.

M Simonyi, I Fitos, Z Tegyey, L Otvös

Index: Biochem. Biophys. Res. Commun. 97(1) , 1-7, (1980)

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Abstract

The continuous exchange of the radioactive free ligand by its non-labelled isotopomer of identical concentration does not affect the position of the overall-equilibrium. The replacement of the enantiomer of higher binding affinity lags behind the replacement of the other one that leads to stereoselective labelling of racemates. Radioactivity, as a function of filtrate volume can be fitted to theoretical expressions. The method allows to study stereoselectivity of binding equilibria set by racemic ligands.