Chirality 1999-01-01

Stereoselective allosteric binding interaction on human serum albumin between ibuprofen and lorazepam acetate.

I Fitos, J Visy, M Simonyi, J Hermansson

Index: Chirality 11(2) , 115-20, (1999)

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Abstract

The effect of ibuprofen enantiomers on the stereoselective binding of 3-acyloxy-1,4-benzodiazepines to human serum albumin (HSA) was studied using both native and Sepharose-immobilized protein. (S)-Lorazepam acetate exhibited considerably enhanced binding, especially in the presence of (+)-(S)-ibuprofen. The phenomenon is an indication of cooperative allosteric interaction between different binding sites during multiple cobinding of two ligands.