Journal of Chromatography B 2015-12-01

Capillary electrophoresis with end-column electrochemiluminescence for ultrasensitive determination of urapidil hydrochloride in rat plasma and its application to pharmacokinetics study.

Shuangjiao Sun, Yanfen Wei, Chanjuan Long, Biyang Deng

Index: J. Chromatogr. B. Analyt. Technol. Biomed. Life Sci. 1006 , 146-50, (2015)

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Abstract

A simple, sensitive and selective method for determination of urapidil hydrochloride was developed using capillary electrophoresis with electrochemiluminescence (CE-ECL) technique for the first time. Under the optimized experimental conditions, the ECL intensity was linear with the concentration of urapidil hydrochloride in the range from 0.050 to 50.0ng/mL and the detection limit was 0.014ng/mL (S/N=3). The proposed method was used for studying pharmacokinetics of urapidil hydrochloride in rat plasma and the main pharmacokinetic parameters of the peak concentration (Cmax), half life time (T1/2) and peak concentration time (Tmax) were 240.45±21.15ng/mL, 0.58±0.16h and 1.08±0.13h, respectively. The recoveries of urapidil hydrochloride in the diluted extracts of rat plasma samples ranged from 96.68 to 98.82%. The RSD was lower than 3%. Copyright © 2015 Elsevier B.V. All rights reserved.