Journal of chromatography. A 2013-01-25

Development of novel molecularly imprinted magnetic solid-phase extraction materials based on magnetic carbon nanotubes and their application for the determination of gatifloxacin in serum samples coupled with high performance liquid chromatography.

Deli Xiao, Pierre Dramou, Nanqian Xiong, Hua He, Hui Li, Danhua Yuan, Hao Dai

Index: J. Chromatogr. A. 1274 , 44-53, (2013)

Full Text: HTML

Abstract

A novel composite imprinted material, on the basis of magnetic carbon nanotubes (MCNTs)-incorporated layer using gatifloxacin as a template, methacrylic acid as a functional monomer, and ethylene glycol dimethacrylate as a cross-linker, was successfully synthesized by a surface imprinting technique. Adsorption dynamics and a Scatchard adsorption model were employed to evaluate the adsorption process. The results showed that magnetic carbon nanotubes molecularly imprinted polymers (MCNTs@MIP) displayed a rapid dynamic adsorption and a high adsorption capacity of 192.7 μg/mg toward GTFX. Applied MCNTs@MIP as a sorbent, a magnetic solid phase extraction method coupled with high performance liquid chromatography (MSPE-HPLC) was developed for the determination of GTFX in serum samples. The recoveries from 79.1±4.8% to 85.3±4.2% were obtained. MCNTs@MIP can not only be collected and separated fast by external magnetic field but also have high surface-to-volume ratio, outstanding mechanical properties and specific recognition toward template molecule. In addition, the MCNTs@MIP could be regenerated, which could be used for five cycles with lost of less than 7.8% of its recovery on average. These analytical results of serum samples display that the proposed method based on MCNTs@MIP is applicable for fast and selective extraction of therapeutic agents from biological fluids.Copyright © 2012 Elsevier B.V. All rights reserved.