Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 1995-10-01

Distributions of nitropyrenes and mutagenicity in airborne particulates collected with an Andersen sampler.

K Hayakawa, Y Kawaguchi, T Murahashi, M Miyazaki

Index: Mutat. Res. 348(2) , 57-61, (1995)

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Abstract

Distributions of 1,3-dinitropyrene (1,3-DNP), 1,6-DNP, 1,8-DNP, 1-nitropyrene (1-NP) and mutagenicity in airborne particulates collected in downtown Kanazawa, Japan with an Andersen high-volume air sampler were examined. Mutagenicities of benzene-ethanol extract from particulates were determined by the Ames test using S. typhimurium strains without S9 mix, while concentrations of DNPs and 1-NP were determined by high-performance liquid chromatography (HPLC) using chemiluminescence detection. In the finest particulate fraction (smaller than 1.1 microns), 68% and 75% of the total mutagenicities were observed in TA98 and YG1024 strains, respectively. In the same fraction, 65-82% of three DNPs as well as 84% of 1-NP were observed. Mutagenic contributions of 1,3-DNP, 1,6-DNP, 1,8-DNP and 1-NP in the extract were respectively 0.6, 1.2, 1.8 and 1.6% in the TA98 strain, and 2.5, 5, 9 and 2.1% in the YG1024 strain.