Applied Biochemistry and Biotechnology 2007-04-01

Reusability of entrapped cells of Pseudomonas diminuta for production of 7-aminocephalosporanic acid.

V K Nigam, S Kundu, P Ghosh

Index: Appl. Biochem. Biotechnol. 141(1) , 119-26, (2007)

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Abstract

Entrapped cells of P. diminuta were used for the production of 7-aminocephalosporanic acid (7-ACA), a key intermediate required for the production of most of the clinically used cephalosporin derivatives, i.e., semisynthetic cephalosporins. The repeated batch production of 7-ACA with entrapped cells of P. diminuta in different carriers were carried out for six cycles at optimal conditions. It was found that 33% , 38%, and 47% of activity was lost with chitosan, gelatin, and agar, respectively as immobilizing supports after the sixth cycle of operation.