Scientific reports 2015-01-01

QUANTITY: An Isobaric Tag for Quantitative Glycomics.

Shuang Yang, Meiyao Wang, Lijun Chen, Bojiao Yin, Guoqiang Song, Illarion V Turko, Karen W Phinney, Michael J Betenbaugh, Hui Zhang, Shuwei Li

Index: Sci. Rep. 5 , 17585, (2015)

Full Text: HTML

Abstract

Glycan is an important class of macromolecules that play numerous biological functions. Quantitative glycomics--analysis of glycans at global level--however, is far behind genomics and proteomics owing to technical challenges associated with their chemical properties and structural complexity. As a result, technologies that can facilitate global glycan analysis are highly sought after. Here, we present QUANTITY (Quaternary Amine Containing Isobaric Tag for Glycan), a quantitative approach that can not only enhance detection of glycans by mass spectrometry, but also allow high-throughput glycomic analysis from multiple biological samples. This robust tool enabled us to accomplish glycomic survey of bioengineered Chinese Hamster Ovary (CHO) cells with knock-in/out enzymes involved in protein glycosylation. Our results demonstrated QUANTITY is an invaluable technique for glycan analysis and bioengineering.