Enzymatic synthesis of 2′-deoxyguanosine with nucleoside deoxyribosyltransferase-II

K Okuyama, S Shibuya, T Hamamoto…

Index: Okuyama, Kiyoshi; Shibuya, Susumu; Hamamoto, Tomoki; Noguchi, Toshitada Bioscience, biotechnology, and biochemistry, 2003 , vol. 67, # 5 p. 989 - 995

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Citation Number: 36

Abstract

Nucleoside deoxyribosyltransferase-II (NdRT-II) of Lactobacillus helveticus, which catalyzes the transfer of a glycosyl residue from a donor deoxyribonucleoside to an acceptor base, has a broad specificity for the acceptor bases. Six-substituted purines were found to be substrates as acceptor bases for NdRT-II. Using this property of the enzyme, we established a practical procedure for enzymatic synthesis of 2′-deoxyguanosine (dGuo), consisting ...