A highly efficient synthesis of homogeneous triglycerides of either pure eicosapentaenoic acid, 1, or docosahexaenoic acid, 2, by an immobilized non-regiospecific yeast lipase from Candida antarctica is described. Two methods were used: Interesterification of tributyrin and direct esterification of glycerol with stoichiometric amount of 99% EPA or DHA as ethyl esters and free fatty acids, respectively. Both processes were performed under vacuum at 65° C ...
