Abstract More than 95% of polyunsaturated acid (PUFA) was converted to triacylglycerol by immobilized lipase from Candida antarctica or Rhizomucor miehei. The esterification was carried out at 50–60° C with shaking and dehydration for 24 h. The substrates consisted of glycerol and free fatty acid or ethyl esters of the fatty acid at a 1∶ 3 molar ratio. The docosahexaenoic acid (DHA) or eicosapentaenoic acid (EPA) in the substrate ...
