24 hr. (210 to 240 ml.) the temperature was raised to 50'. A linear gradient system going from the 0.2 N, @H 3.02 buffer to a 2 N, pH 5.09 sodium citrate-acetate buffer (500 ml. of each) was started after 34 to 38 hours (340 to 380 ml.). A total of 1000 mi. of buffer was used to develop the columns. The colorimetric detection procedure'g" made use of the color reaction given by amino acids and ammonia with ninhydrin. The optical density of the solutions was ...
