• We developed an epitope imprinting approach for pTyr peptide capture by MIPs. • PPA, a characteristic component of phenylphosphate group was used as template. • MIPs showed good selectivity and clear preference for pTyr peptide over pSer. • MIPs performed much more capacity (1.5-fold) to TiO2 for pTyr peptide recognition. • After three reuse cycles, there were still 94% of adsorption capacity retained.
