Pharmaceutical Biology 2015-03-01

Chemical profile and biological activities of Veronica thymoides subsp. pseudocinerea.

Abdulselam Ertas, Mehmet Boga, Murat Kizil, Bircan Ceken, Ahmet Ceyhan Goren, Nesrin Hasimi, Serpil Demirci, Gulacti Topcu, Ufuk Kolak

文献索引:Pharm. Biol. 53(3) , 334-9, (2015)



In Turkey, Veronica species (Plantaginaceae) have been used as a diuretic and for wound healing in traditional medicine.To examine the fatty acid and essential oil profiles, the antioxidant, anticholinesterase, antimicrobial, and DNA damage effects of Veronica thymoides P.H. Davis subsp. pseudocinerea M.A. Fischer as a potential source of natural active compounds.GC/MS was used to analyze essential oil and fatty acid obtained from whole plant. The antioxidant activity was evaluated by the β-carotene-linoleic acid test system, DPPH-free and ABTS cation radicals scavenging, and cupric reducing antioxidant capacity assays. The anticholinesterase and antimicrobial activities were determined by Ellman and broth macrodillution methods, respectively. The effect of the methanol extract on DNA cleavage was investigated.Hexatriacontene (21.0%) was found to be the main constituent in essential oil, and linoleic acid (25.2%) and palmitic acid (20.6%) in fatty acid. Methanol extract demonstrated the best IC50 values in lipid peroxidation (49.81 ± 0.31 µg/ml) and DPPH-free radical scavenging activity (15.32 ± 0.17 µg/ml). Methanol and water extracts possessed strong ABTS cation radical scavenging activity with IC50 values 9.15 ± 0.28 and 8.90 ± 0.1  µg/ml, respectively. The acetone extract exhibited moderate butyrylcholinesterase inhibitory activity. The highest antimicrobial activity was determined in methanol extract against Escherichia coli with 31.25 µg/ml MIC value. Inhibition of methanol extract on plasmid DNA cleavage by OH radicals was found to be 93.32% at 500 µg/ml.The methanol extract having strong antioxidant and DNA damage effects could be investigated phytochemically to find natural active compounds.


结构式 名称/CAS号 全部文献
硫酸 结构式 硫酸
无水醋酸钠 结构式 无水醋酸钠
碳酸钠 结构式 碳酸钠
丙酮 结构式 丙酮
氯仿 结构式 氯仿
乙醇 结构式 乙醇
乙醚 结构式 乙醚
正己烷 结构式 正己烷
乙酸铵 结构式 乙酸铵
双氧水 结构式 双氧水