China Journal of Chinese Materia Medica 2006-02-01

[Determination of acetylharpagide in Ajuga decumbens by HPLC].

Dang-Kun Yue, Guang-Zhi Ding, Wei-Hao Wang, Zhi-Min Wang, Jian-Rong Li, Yan Tong

文献索引：Zhongguo Zhong Yao Za Zhi 31(4) , 289-90, (2006)

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摘要

To establish a method of the quantitative determination of acetylharpagide in Ajuga decumbens.The chromatographic conditions were as follows: a Phenomenex Luna C18 column was used, the mobile phase was composed of acetontrile-water (15:85), the flow rate was 1.0 mL x min(-1) and the UV absorbance detection was set at 197 nm.Linearity of acetylharpagide was in the range of 0.6-3.6 microg (r = 0.9993), and the average recovery and RSD were 99.13% and 2.48%, respectively.The contents of acetylharpagide ranged 0.40%-6.39% in A. decumbens. The method was simple, accurate and sensitive.