Analytica Chimica Acta 2014-10-03

Aptamer-functionalized solid phase microextraction-liquid chromatography/tandem mass spectrometry for selective enrichment and determination of thrombin.

Fuyou Du, Md Nazmul Alam, Janusz Pawliszyn

文献索引:Anal. Chim. Acta 845 , 45-52, (2014)



In this publication, a novel solid phase microextraction (SPME) coating functionalized with a DNA aptamer for selective enrichment of a low abundance protein from diluted human plasma is described. This approach is based on the covalent immobilization of an aptamer ligand on electrospun microfibers made with the hydrophilic polymer poly(acrylonitrile-co-maleic acid) (PANCMA) on stainless steel rods. A plasma protein, human α-thrombin, was employed as a model protein for selective extraction by the developed Apt-SPME probe, and the detection was carried out with liquid chromatography/tandem mass spectrometry (LC-MS/MS). The SPME probe exhibited highly selective capture, good binding capacity, high stability and good repeatability for the extraction of thrombin. The protein selective probe was employed for direct extraction of thrombin from 20-fold diluted human plasma samples without any other purification. The Apt-SPME method coupled with LC-MS/MS provided a good linear dynamic range of 0.5-50 nM in diluted human plasma with a good correlation coefficient (R(2)=0.9923), and the detection limit of the proposed method was found to be 0.30 nM. Finally, the Apt-SPME coupled with LC-MS/MS method was successfully utilized for the determination of thrombin in clinical human plasma samples. One shortcoming of the method is its reduced efficiency in undiluted human plasma compared to the standard solution. Nevertheless, this new aptamer affinity-based SPME probe opens up the possibility of selective enrichment of a given targeted protein from complex sample either in vivo or ex vivo.Copyright © 2014 Elsevier B.V. All rights reserved.


结构式 名称/CAS号 全部文献
甲酸 结构式 甲酸
乙腈 结构式 乙腈
亚硫酸钠 结构式 亚硫酸钠
甲醇 结构式 甲醇
二甲基甲酰胺 结构式 二甲基甲酰胺
顺丁烯二酸酐 结构式 顺丁烯二酸酐
丙烯腈 结构式 丙烯腈
诺卜醇 结构式 诺卜醇
N-羟基琥珀酰亚胺 结构式 N-羟基琥珀酰亚胺
过硫酸钾 结构式 过硫酸钾