Proofing of photolithographic DNA synthesis with 3', 5'-dimethoxybenzoinyloxycarbonyl-protected deoxynucleoside phosphoramidites

MC Pirrung, L Fallon, G McGall

文献索引：Pirrung, Michael C.; Fallon, Lara; McGall, Glenn Journal of Organic Chemistry, 1998 , vol. 63, # 2 p. 241 - 246

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被引用次数: 114

摘要

We have evaluated in a microchip format the photochemical solid-phase phosphoramidite DNA synthesis method we previously developed. A set of nucleoside building blocks with “easy-off” base protecting groups was prepared bearing photolabile 5'-O- dimethoxybenzoincarbonate (DMBOC) groups. Photolysis rates and cycle yields for these DMBOC-protected nucleotides covalently attached to planar, derivatized glass surfaces ...