Human serum albumin binding assay based on displacement of a non selective fluorescent inhibitor

A Thorarensen, RW Sarver, F Tian, A Ho…

文献索引：Thorarensen, Atli; Sarver, Ronald W.; Tian, Fang; Ho, Andrea; Romero, Donna L.; Marotti, Keith R. Bioorganic and Medicinal Chemistry Letters, 2007 , vol. 17, # 16 p. 4646 - 4649

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被引用次数: 7

摘要

In this paper, we describe a fluorescent antibacterial analog, 6, with utility as a competition probe to determine affinities of other antibacterial analogs for human serum albumin (HSA). Analog 6 bound to HSA with an affinity of 400±100nM and the fluorescence was environmentally sensitive. With 370nm excitation, environmental sensitivity was indicated by a quenching of the 530nm emission when the probe bound to HSA. Displacement of ...