Antibody??Catalyzed Removal of the p??Nitrobenzyl Ester Protecting Group: The Molecular Basis of Broad Substrate Specificity

S Kurihara, T Tsumuraya, K Suzuki…

文献索引：Kurihara, Shinwa; Tsumuraya, Takeshi; Suzuki, Kayo; Kuroda, Masataka; Liu, Lidong; Takaoka, Yumiko; Fujii, Ikuo Chemistry - A European Journal, 2000 , vol. 6, # 9 p. 1656 - 1662

全文：HTML全文

被引用次数: 20

摘要

Abstract Antibody catalysts for the removal of the p-nitrobenzyl ester protecting group have been generated to accommodate a broad range of substrates. Antibody 7B9, which was elicited against p-nitrobenzyl phosphonate 1, catalyzed the hydrolyses of p-nitrobenzyl monoesters of nonsubstituted, and β-and γ-substituted glutaric acids with almost identical K m and k cat values. In addition, 7B9 displayed substrate tolerance towards the α- ...